Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NaCl | enzyme shows its maximum hydrolysis activity in a buffer with 1.5 M NaCl and retains up to 65% of its activity at 0.5 M NaCl | Haloferax mediterranei |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Haloferax mediterranei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km value is 17.2 mg/ml for beta-cyclization activity using soluble starch, pH 7.0, 55°C | Haloferax mediterranei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | secretion by the Tat pathway | Haloferax mediterranei | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
82000 | - |
x * 82000, SDS-PAGE | Haloferax mediterranei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haloferax mediterranei | Q53I75 | - |
- |
Purification (Comment) | Organism |
---|---|
native enzyme | Haloferax mediterranei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
48 | - |
pH 7.5, 55°C | Haloferax mediterranei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | cyclization is the predominant activity, followed by hydrolysis and to a lesser extent coupling and disproportionation activities | Haloferax mediterranei | ? | - |
? | |
soluble potato starch | - |
Haloferax mediterranei | alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin | ratio of products is alpha-cyclodextrin to beta-cyclodextrin to gamma-cyclodextrin 1:0.6:0.3 | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 82000, SDS-PAGE | Haloferax mediterranei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
presence of 1.5 M NaCl | Haloferax mediterranei |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
50% of maximum activity | Haloferax mediterranei |
60 | - |
50% of maximum activity | Haloferax mediterranei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
presence of 1.5 M NaCl | Haloferax mediterranei |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | loss of more than 60% of activity above or below | Haloferax mediterranei |