Literature summary for 2.4.1.19 extracted from

Rostinawati, T.; Riani, C.; Elfahmi, E.; Sumirtapura, Y.; Retnoningrum, D.
Y93F substitution of cyclodextrin glucanotransferase from Bacillus sp. A2-5a and its enzyme characterization (2015), Biotechnology, 14, 181-187.

No PubMed abstract available

Search for more literature for 2.4.1.19

Cloned(Commentary)

Cloned (Comment)	Organism
mutant enzyme Y93F is expressed in Escherichia coli BL21(DE3) cells	Bacillus sp. (in: Bacteria)

Protein Variants

Protein Variants	Comment	Organism
Y93F	the substitution causes no alpha-cyclodextrin formation, but produces 6% more beta-cyclodextrin, 16% less gamma-cyclodextrin, and decreases its kcat and kcat/Km values	Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
76390	-	x * 76390, SDS-PAGE	Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
soluble starch + glycosyl acceptor	Bacillus sp. (in: Bacteria)	-	cyclodextrins	-	?
soluble starch + glycosyl acceptor	Bacillus sp. (in: Bacteria) A2-5a	-	cyclodextrins	-	?

Organism

Organism	UniProt	Comment	Textmining
Bacillus sp. (in: Bacteria)	-	-	-
Bacillus sp. (in: Bacteria) A2-5a	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA resin column chromatography	Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
soluble starch + glycosyl acceptor	-	Bacillus sp. (in: Bacteria)	cyclodextrins	-	?
soluble starch + glycosyl acceptor	-	Bacillus sp. (in: Bacteria) A2-5a	cyclodextrins	-	?

Subunits

Subunits	Comment	Organism
?	x * 76390, SDS-PAGE	Bacillus sp. (in: Bacteria)

Synonyms

Synonyms	Comment	Organism
CGTase	-	Bacillus sp. (in: Bacteria)
cyclodextrin glucanotransferase	-	Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
60	-	-	Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Bacillus sp. (in: Bacteria)

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Release 2023.1 (January 2023)