Cloned (Comment) | Organism |
---|---|
mutant Y195I is expressed in Escherichia coli BL21(DE3) cells | Paenibacillus macerans |
Crystallization (Comment) | Organism |
---|---|
mutant Y195I, hanging drop vapor diffusion method, using 0.2 M sodium acetate trihydrate, 0.1 M Tris hydrochloride pH 9.0, 30% (w/v) polyethylene glycol 4000, at 22°C | Paenibacillus macerans |
Protein Variants | Comment | Organism |
---|---|---|
Y195I | the mutation drastically alters the cyclodextrin specificity of the enzyme by switching toward the synthesis of both beta- and gamma-cyclodextrins | Paenibacillus macerans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + glycosyl acceptor | Paenibacillus macerans | - |
cyclodextrins | the main product is alpha-cyclodextrin | ? | |
starch + glycosyl acceptor | Paenibacillus macerans 602-1 | - |
cyclodextrins | the main product is alpha-cyclodextrin | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenibacillus macerans | - |
- |
- |
Paenibacillus macerans 602-1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography and Sephacryl-100 gel filtration | Paenibacillus macerans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + glycosyl acceptor | - |
Paenibacillus macerans | cyclodextrins | the main product is alpha-cyclodextrin | ? | |
starch + glycosyl acceptor | - |
Paenibacillus macerans 602-1 | cyclodextrins | the main product is alpha-cyclodextrin | ? |
Synonyms | Comment | Organism |
---|---|---|
alpha-CGTase | - |
Paenibacillus macerans |
alpha-cyclodextrin glycosyltransferase | - |
Paenibacillus macerans |