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Literature summary for 2.4.1.198 extracted from

  • Engel, J.; Schmalhorst, P.S.; Krueger, A.T.; Mueller, C.T.; Buettner, F.F.; Routier, F.H.
    Characterization of an N-acetylglucosaminyltransferase involved in Aspergillus fumigatus zwitterionic glycoinositolphosphoceramide biosynthesis (2015), Glycobiology, 25, 1423-1430.
    View publication on PubMed
Search for more literature for 2.4.1.198

Cloned(Commentary)

Cloned (Comment) Organism
gene gtA, functional recombinant expression of FLAG-tagged GntA enzyme in Saccharomyces cerevisiae Aspergillus fumigatus

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi apparatus
-
 Aspergillus fumigatus 5794
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 x * 40000, recombinant FLAG-tagged GntA, SDS-PAGE Aspergillus fumigatus

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus
-
 gene gntA
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein two putative N-glycosylation sites at Asn66 and Asn243. The two predicted N-glycosylation sites are located on each side of the catalytically relevant DXD motif Aspergillus fumigatus

Subunits

Subunits Comment Organism
? x * 40000, recombinant FLAG-tagged GntA, SDS-PAGE Aspergillus fumigatus

Synonyms

Synonyms Comment Organism
GntA
-
 Aspergillus fumigatus

General Information

General Information Comment Organism
malfunction targeted deletion of the gene encoding GntA in A. fumigatus results in complete absence of zwitterionic glycoinositolphosphoceramide, a phenotype that can be reverted by episomal expression of GntA in the mutant Aspergillus fumigatus
physiological function the enzyme is involved in the initiation of zwitterionic glycoinositolphosphoceramide biosynthesis. Glycoinositolphosphoceramides (GIPCs) are complex sphingolipids that, in fungi, commonly contain an alpha-mannose residue linked at position 2 of the inositol. Several pathogenic fungi additionally synthesize zwitterionic GIPCs carrying an alpha-glucosamine residue at this position. In the human pathogen Aspergillus fumigatus, the alpha-N-acetylglucosaminyl-1,2-inositolphosphoceramide core is elongated to Manalpha1,3Manalpha1,6GlcNalpha1,2IPC, which is the most abundant glycoinositolphosphoceramide synthesized by this fungus. GntA uses UDP-N-acetylglucosamine as donor substrate to generate a glycolipid product resistant to saponification and to digestion by phosphatidylinositol-phospholipase C as expected for GlcNAcalpha1,2IPC Aspergillus fumigatus