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Literature summary for 2.4.1.20 extracted from

  • Sasaki, T.
    Cellobiose phosphorylase from Cellovibrio gilvus (1988), Methods Enzymol., 160, 468-472.
No PubMed abstract available

General Stability

General Stability Organism
storage of purified enzyme at -20°C without thiol reagents causes rapid loss of activity Cellulomonas gilvus

Inhibitors

Inhibitors Comment Organism Structure
alpha-oxogluconate
-
Cellulomonas gilvus
glucono-delta-lactone very weak Cellulomonas gilvus
N-ethylmaleimide 56% inhibition at 0.5 mM Cellulomonas gilvus
Nojirimycin competitive with respect to cellobiose Cellulomonas gilvus
p-chloromercuribenzoate complete inhibition at 0.02 mM Cellulomonas gilvus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.77
-
phosphate
-
Cellulomonas gilvus
1.25
-
cellobiose
-
Cellulomonas gilvus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Cellulomonas gilvus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72000
-
SDS-PAGE Cellulomonas gilvus
280000
-
gel filtration Cellulomonas gilvus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cellobiose + phosphate Cellulomonas gilvus
-
alpha-D-glucose 1-phosphate + D-glucose
-
?

Organism

Organism UniProt Comment Textmining
Cellulomonas gilvus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
use of thiol reagent necessary during purification to maintain activity Cellulomonas gilvus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
27.4
-
purified enzyme Cellulomonas gilvus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose
-
Cellulomonas gilvus ?
-
?
alpha-D-glucose 1-phosphate + 6-deoxy-D-glucose
-
Cellulomonas gilvus ?
-
?
alpha-D-glucose 1-phosphate + D-altrose
-
Cellulomonas gilvus ?
-
?
alpha-D-glucose 1-phosphate + D-arabinose
-
Cellulomonas gilvus ?
-
?
alpha-D-glucose 1-phosphate + D-glucosamine
-
Cellulomonas gilvus ?
-
?
alpha-D-glucose 1-phosphate + D-mannose
-
Cellulomonas gilvus ?
-
?
alpha-D-glucose 1-phosphate + D-xylose
-
Cellulomonas gilvus ?
-
?
alpha-D-glucose 1-phosphate + L-fucose
-
Cellulomonas gilvus ?
-
?
alpha-D-glucose 1-phosphate + L-galactose
-
Cellulomonas gilvus ?
-
?
cellobiose + phosphate
-
Cellulomonas gilvus alpha-D-glucose 1-phosphate + D-glucose
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 72000, gel filtration, SDS-PAGE Cellulomonas gilvus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
inactivated above Cellulomonas gilvus
60
-
complete inactivation within 10 min Cellulomonas gilvus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
-
Cellulomonas gilvus