Literature summary for 2.4.1.20 extracted from

Kim, Y.K.; Kitaoka, M.; Krishnareddy, M.; Mori, Y.; Hayashi, K.
Kinetic studies of a recombinant cellobiose phosphorylase (CBP) of the Clostridium thermocellum YM4 strain expressed in Escherichia coli (2002), J. Biochem., 132, 197-203.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Acetivibrio thermocellus

Inhibitors

Inhibitors	Comment	Organism	Structure
D-glucose	substrate inhibition, competitive with glucose-1-phosphate	Acetivibrio thermocellus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.97	-	D-glucose	-	Acetivibrio thermocellus
5.9	-	D-glucosamine	-	Acetivibrio thermocellus
6.1	-	6-deoxy-D-glucose	-	Acetivibrio thermocellus
21.4	-	D-xylose	-	Acetivibrio thermocellus
56.9	-	2-deoxy-D-glucose	-	Acetivibrio thermocellus
131	-	D-mannose	-	Acetivibrio thermocellus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
92000	-	SDS-PAGE, native PAGE	Acetivibrio thermocellus
93760	-	calculated from DNA-sequence	Acetivibrio thermocellus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
cellobiose + phosphate	Acetivibrio thermocellus	-	alpha-D-glucose 1-phosphate + D-glucose	-	?
cellobiose + phosphate	Acetivibrio thermocellus YM4	-	alpha-D-glucose 1-phosphate + D-glucose	-	?

Organism

Organism	UniProt	Comment	Textmining
Acetivibrio thermocellus	-	-	-
Acetivibrio thermocellus YM4	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme from E. coli	Acetivibrio thermocellus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose	-	Acetivibrio thermocellus	?	-	?
alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose	-	Acetivibrio thermocellus YM4	?	-	?
alpha-D-glucose 1-phosphate + 6-deoxy-D-glucose	-	Acetivibrio thermocellus	?	-	?
alpha-D-glucose 1-phosphate + 6-deoxy-D-glucose	-	Acetivibrio thermocellus YM4	?	-	?
alpha-D-glucose 1-phosphate + D-glucosamine	-	Acetivibrio thermocellus	?	-	?
alpha-D-glucose 1-phosphate + D-mannose	-	Acetivibrio thermocellus	?	-	?
alpha-D-glucose 1-phosphate + D-xylose	no activity with L-isomer	Acetivibrio thermocellus	?	-	?
cellobiose + phosphate	-	Acetivibrio thermocellus	alpha-D-glucose 1-phosphate + D-glucose	-	?
cellobiose + phosphate	ordered bi-bi mechanism	Acetivibrio thermocellus	alpha-D-glucose 1-phosphate + D-glucose	-	?
cellobiose + phosphate	beta-cellobiose preferred over alpha-anomer	Acetivibrio thermocellus	alpha-D-glucose 1-phosphate + D-glucose	-	?
cellobiose + phosphate	-	Acetivibrio thermocellus YM4	alpha-D-glucose 1-phosphate + D-glucose	-	?
cellobiose + phosphate	ordered bi-bi mechanism	Acetivibrio thermocellus YM4	alpha-D-glucose 1-phosphate + D-glucose	-	?
cellobiose + phosphate	beta-cellobiose preferred over alpha-anomer	Acetivibrio thermocellus YM4	alpha-D-glucose 1-phosphate + D-glucose	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 92000, SDS-PAGE, native PAGE	Acetivibrio thermocellus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
7.7	-	D-glucosamine	-	Acetivibrio thermocellus
9.3	-	D-mannose	-	Acetivibrio thermocellus
12.2	-	D-glucose	-	Acetivibrio thermocellus
18.3	-	2-deoxy-D-glucose	-	Acetivibrio thermocellus
44.3	-	D-xylose	-	Acetivibrio thermocellus
59.5	-	6-deoxy-D-glucose	-	Acetivibrio thermocellus

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Release 2023.1 (January 2023)