Literature summary for 2.4.1.20 extracted from

Ohdan, K.; Fujii, K.; Yanase, M.; Takaha, T.; Kuriki, T.
Phosphorylase coupling as a tool to convert cellobiose into amylose (2007), J. Biotechnol., 127, 496-502.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli strain BL21 (DE3)	Acetivibrio thermocellus

Organism

Organism	UniProt	Comment	Textmining
Acetivibrio thermocellus	-	strain YM4 (AB013109). To convert cellobiose into amylose: the cellobiose phosphorylase catalyzes phosphorolysis of cellobiose into G1-P and glucose, then alpha-glucan phosphorylase catalyses the polymerization of alpha-1,4-glucan, using G1-P as a carbohydrate donor and maltotetraose as the initial carbohydrate acceptor. Thus, glucosyl moieties of cellobiose molecules are successively transferred to the non-reducing ends of glucan to elongate the chain. The optimal conditions are: thirty milligrams per milliliters (87.7 mM) cellobiose, 30 mM sodium phosphate (pH 7.0), 30 microg/ml cellobiose phosphorylase, 30 microg/ml alpha-glucan phosphorylase, and 75 microM maltotetraose are incubated at 45°C	-
Acetivibrio thermocellus YM4	-	strain YM4 (AB013109). To convert cellobiose into amylose: the cellobiose phosphorylase catalyzes phosphorolysis of cellobiose into G1-P and glucose, then alpha-glucan phosphorylase catalyses the polymerization of alpha-1,4-glucan, using G1-P as a carbohydrate donor and maltotetraose as the initial carbohydrate acceptor. Thus, glucosyl moieties of cellobiose molecules are successively transferred to the non-reducing ends of glucan to elongate the chain. The optimal conditions are: thirty milligrams per milliliters (87.7 mM) cellobiose, 30 mM sodium phosphate (pH 7.0), 30 microg/ml cellobiose phosphorylase, 30 microg/ml alpha-glucan phosphorylase, and 75 microM maltotetraose are incubated at 45°C	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA agarose slurry, Superdex 200 column and Mono Q column	Acetivibrio thermocellus

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Release 2023.1 (January 2023)