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Literature summary for 2.4.1.20 extracted from

  • Ye, X.; Zhang, C.; Zhang, Y.H.
    Engineering a large protein by combined rational and random approaches: stabilizing the Clostridium thermocellum cellobiose phosphorylase (2012), Mol. Biosyst., 8, 1815-1823.
    View publication on PubMed
Search for more literature for 2.4.1.20

Protein Variants

Protein Variants Comment Organism
A423S mutation introduced to increase thermostability, mutant displays a half-life of 11.3 min at 70°C Acetivibrio thermocellus
A781K mutation introduced to increase thermostability, mutant displays a half-life of 15.3 min at 70°C Acetivibrio thermocellus
Q130H/K131Y/S411G/A423S/A781K mutations introduced to increase thermostability, mutant displays a half-life of 17.7 min at 70°C Acetivibrio thermocellus
R48R/Q130H/K131Y/K142R/S411G/A423S/V526A/A781K mutations introduced to increase thermostability, mutant displays a half-life of 24.6 min at 70°C Acetivibrio thermocellus
S411G mutation introduced to increase thermostability, mutant displays a half-life of 13.6 min at 70°C Acetivibrio thermocellus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.9
-
 cellobiose mutant S411G, pH 7.2, 40°C Acetivibrio thermocellus
10.3
-
 cellobiose mutant A423S, pH 7.2, 40°C Acetivibrio thermocellus
11
-
 cellobiose mutant A781 K, pH 7.2, 40°C Acetivibrio thermocellus
14.7
-
 cellobiose mutant R48R/Q130H/K131Y/K142R/S411G/A423S/V526A/A781K, pH 7.2, 40°C Acetivibrio thermocellus
15.9
-
 cellobiose mutant Q130H/K131Y/S411G/A423S/A781K, pH 7.2, 40°C Acetivibrio thermocellus
16.5
-
 cellobiose wild-type, pH 7.2, 40°C Acetivibrio thermocellus

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + phosphate
-
 Acetivibrio thermocellus D-glucose 1-phosphate + D-glucose
-
 ?

Synonyms

Synonyms Comment Organism
Cbp
-
 Acetivibrio thermocellus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 wild-type Acetivibrio thermocellus
80
-
 mutant R48R/Q130H/K131Y/K142R/S411G/A423S/V526A/A781K Acetivibrio thermocellus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 half-life of wild-type 8.3 min, mutant Q130H/K131Y/S411G/A423S/A781K 17.7 min, mutant R48R/Q130H/K131Y/K142R/S411G/A423S/V526A/A781K 24.6 min Acetivibrio thermocellus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3
-
 cellobiose mutant S411G, pH 7.2, 40°C Acetivibrio thermocellus
1.5
-
 cellobiose mutant R48R/Q130H/K131Y/K142R/S411G/A423S/V526A/A781K, pH 7.2, 40°C Acetivibrio thermocellus
2.1
-
 cellobiose mutant Q130H/K131Y/S411G/A423S/A781K, pH 7.2, 40°C Acetivibrio thermocellus
2.9
-
 cellobiose mutant A423S, pH 7.2, 40°C Acetivibrio thermocellus
2.9
-
 cellobiose mutant A781 K, pH 7.2, 40°C Acetivibrio thermocellus
3.4
-
 cellobiose wild-type, pH 7.2, 40°C Acetivibrio thermocellus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.103
-
 cellobiose mutant R48R/Q130H/K131Y/K142R/S411G/A423S/V526A/A781K, pH 7.2, 40°C Acetivibrio thermocellus
0.134
-
 cellobiose mutant Q130H/K131Y/S411G/A423S/A781K, pH 7.2, 40°C Acetivibrio thermocellus
0.189
-
 cellobiose mutant S411G, pH 7.2, 40°C Acetivibrio thermocellus
0.2
-
 cellobiose wild-type, pH 7.2, 40°C Acetivibrio thermocellus
0.263
-
 cellobiose mutant A781 K, pH 7.2, 40°C Acetivibrio thermocellus
0.287
-
 cellobiose mutant A423S, pH 7.2, 40°C Acetivibrio thermocellus