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Literature summary for 2.4.1.20 extracted from

  • De Winter, K.; Van Renterghem, L.; Wuyts, K.; Pelantova, H.; Kren, V.; Soetaert, W.; Desmet, T.
    Chemoenzymatic synthesis of beta-D-glucosides using cellobiose phosphorylase from Clostridium thermocellum (2015), Adv. Synth. Catal., 357, 1961-1969.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JW0987 cells Acetivibrio thermocellus

General Stability

General Stability Organism
enzyme immobilization with cross-linked enzyme aggregates boosts the half-life at 50°C from 34 h to almost 11 days Acetivibrio thermocellus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cellobiose + phosphate Acetivibrio thermocellus
-
alpha-D-glucose 1-phosphate + D-glucose
-
r
cellobiose + phosphate Acetivibrio thermocellus YM4
-
alpha-D-glucose 1-phosphate + D-glucose
-
r

Organic Solvent Stability

Organic Solvent Comment Organism
Ethanol ethanol reduces the time required to reduce the initial enzymatic activity by 50% at 50°C from over 32 h to less than 1 h Acetivibrio thermocellus
Ethyl acetate the enzyme displays a relative activity of 92% in 37.5% (v/v) ethyl acetate Acetivibrio thermocellus
Methanol methanol reduces the time required to reduce the initial enzymatic activity by 50% at 50°C from over 32 h to less than 4 h Acetivibrio thermocellus

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus
-
-
-
Acetivibrio thermocellus YM4
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Acetivibrio thermocellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + phosphate
-
Acetivibrio thermocellus alpha-D-glucose 1-phosphate + D-glucose
-
r
cellobiose + phosphate
-
Acetivibrio thermocellus YM4 alpha-D-glucose 1-phosphate + D-glucose
-
r

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37 60 in contrast to the rapid inactivation at 60°C, the enzyme is remarkably stable at 37°C. At 50°C, the enzyme retains over 58% of its initial activity after 24 h Acetivibrio thermocellus