Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JW0987 cells | Acetivibrio thermocellus |
General Stability | Organism |
---|---|
enzyme immobilization with cross-linked enzyme aggregates boosts the half-life at 50°C from 34 h to almost 11 days | Acetivibrio thermocellus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + phosphate | Acetivibrio thermocellus | - |
alpha-D-glucose 1-phosphate + D-glucose | - |
r | |
cellobiose + phosphate | Acetivibrio thermocellus YM4 | - |
alpha-D-glucose 1-phosphate + D-glucose | - |
r |
Organic Solvent | Comment | Organism |
---|---|---|
Ethanol | ethanol reduces the time required to reduce the initial enzymatic activity by 50% at 50°C from over 32 h to less than 1 h | Acetivibrio thermocellus |
Ethyl acetate | the enzyme displays a relative activity of 92% in 37.5% (v/v) ethyl acetate | Acetivibrio thermocellus |
Methanol | methanol reduces the time required to reduce the initial enzymatic activity by 50% at 50°C from over 32 h to less than 4 h | Acetivibrio thermocellus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetivibrio thermocellus | - |
- |
- |
Acetivibrio thermocellus YM4 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Acetivibrio thermocellus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + phosphate | - |
Acetivibrio thermocellus | alpha-D-glucose 1-phosphate + D-glucose | - |
r | |
cellobiose + phosphate | - |
Acetivibrio thermocellus YM4 | alpha-D-glucose 1-phosphate + D-glucose | - |
r |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 60 | in contrast to the rapid inactivation at 60°C, the enzyme is remarkably stable at 37°C. At 50°C, the enzyme retains over 58% of its initial activity after 24 h | Acetivibrio thermocellus |