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Literature summary for 2.4.1.201 extracted from

  • Taguchi, T.; Ogawa, T.; Inoue, S.; Inoue, Y.; Sakamoto, Y.; Korkane, H.; Taniguchi, N.
    Purification and characterization of UDP-GlcNAc:GlcNAcbeta1-6(GlcNAcbeta1-2)Manalpha1-R [GlcNAc to Man]-beta1,4-N-acetylglucosaminyltransferase VI from hen oviduct (2000), J. Biol. Chem., 175, 32598-32602.
No PubMed abstract available
Search for more literature for 2.4.1.201

General Stability

General Stability Organism
dithiothreitol is required for stabilization of the purified enzyme Gallus gallus
enzyme becomes more stable after elution from a Ni2+-chelating column during purification Gallus gallus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Gallus gallus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 8.5% activity compared to Mn2+ Gallus gallus
Co2+ can substitute partially for Mn2+ Gallus gallus
Co2+ 80% activity compared to Mn2+ Gallus gallus
Mg2+ can substitute partially for Mn2+ Gallus gallus
Mg2+ 60% activity compared to Mn2+ Gallus gallus
Mn2+ maximal activity at 5 mM Gallus gallus
Mn2+ absolute requirement Gallus gallus
additional information requirement for divalent cations, no effect of Zn2+, Fe2+, Cu2+ Gallus gallus
Ni2+ can substitute partially for Mn2+ Gallus gallus
Ni2+ 27% activity compared to Mn2+ Gallus gallus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 x * 60000, reducing SDS-PAGE Gallus gallus
72000
-
 x * 72000, non-reducing SDS-PAGE Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R Gallus gallus biosynthesis of pentaantennary asparagine-linked N-glycans UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-R
-
 ?

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
 hen
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Gallus gallus

Purification (Commentary)

Purification (Comment) Organism
-
 Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
oviduct
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.032
-
 purified enzyme Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R acceptor substrate specificity Gallus gallus UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-R
-
 ?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R R represents the remainder of the N-oligosaccharide core of asparagine-linked glycopeptides, (+/-)-fucose, minimum structure requirement: N-acetyl-D-glucosaminyl-beta-1,6-(N-acetylglucosaminyl-beta-1,2)-alpha-mannosyl-R Gallus gallus UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-R
-
 ?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R biantennary oligosaccharides, i.e. Gn T I and II reaction products, are no substrates Gallus gallus UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-R
-
 ?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R biosynthesis of pentaantennary asparagine-linked N-glycans Gallus gallus UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-R
-
 ?

Subunits

Subunits Comment Organism
? x * 72000, non-reducing SDS-PAGE Gallus gallus
? x * 60000, reducing SDS-PAGE Gallus gallus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
 Gallus gallus

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
 broad range Gallus gallus