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Literature summary for 2.4.1.205 extracted from
- Biosynthesis of galactogen: identification of a beta-(1->6)-D-galactosyltransferase in Helix pomatia albumen glands (1989), Biochim. Biophys. Acta, 992, 289-297.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.36 | - |
UDP-D-galactose | - |
Helix pomatia |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | endomembrane-associated, probably in Golgi complex | Helix pomatia | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| MgCl2 | 10 mM, 30% stimulation | Helix pomatia | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
x * 70000, SDS-PAGE | Helix pomatia |
| 600000 | - |
greater than 600000 Da, gel filtration | Helix pomatia |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDPgalactose + galactogen | Helix pomatia | biosynthesis of galactogen | UDP + 1,6-beta-D-galactosylgalactogen | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Helix pomatia | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Helix pomatia |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| albumen gland | - |
Helix pomatia | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.466 | - |
- |
Helix pomatia |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, crude homogenate, 6 months | Helix pomatia |
| 0°C, 24 h, 50% loss of activity | Helix pomatia |
| 4°C, purified enzyme, stable for 1 week | Helix pomatia |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-D-galactose + arabinogalactan | arabinogalactan from larch wood, 22% as effective as galactogen from Helix pomatia | Helix pomatia | UDP + 1,6-beta-D-galactosylarabinogalactan | - |
? | |
| UDP-D-galactose + galactan | galactan from bovine lung 64% as effective as galactogen from Helix pomatia | Helix pomatia | UDP + 1,6-beta-D-galactosylgalactan | - |
? | |
| UDP-D-galactose + galactogen | galactogen from Helix pomatia best acceptor, galactogen from Lymnaea stagnalis, 22% as effective as galactogen from Helix pomatia | Helix pomatia | UDP + 1,6-beta-D-galactosylgalactogen | - |
? | |
| UDPgalactose + galactogen | biosynthesis of galactogen | Helix pomatia | UDP + 1,6-beta-D-galactosylgalactogen | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 70000, SDS-PAGE | Helix pomatia |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 7.5 | - |
Helix pomatia |
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