Any feedback?
Literature summary for 2.4.1.207 extracted from
- The Arabidopsis TCH4 xyloglucan endotransglycosylase. Substrate specificity, pH optimum, and cold tolerance (1997), Plant Physiol., 115, 181-190.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| bovine serum albumin | enhances activity by stabilizing TCH4 protein conformation | Arabidopsis thaliana | |
| additional information | TCH4 protein is not activated by reducing agents, spermidine, spermine, glycerol or carboxymethylcellulose | Arabidopsis thaliana | |
| Urea | enhances activity of TCH4 protein | Arabidopsis thaliana |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloning and expression of TCH4 in Escherichia coli | Arabidopsis thaliana |
| TCH4 is a member of the XET-related gene family | Arabidopsis thaliana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | recombinant TCH4 protein, Km: 0.62 mg/ml for nonfucosylated tamarind xyloglucan as donor, 1.8 mg/ml for fucosylated pea xyloglucan | Arabidopsis thaliana | |
| 0.0003 | - |
xyloglucan acceptor | recombinant TCH4 protein | Arabidopsis thaliana | |
| 0.073 | - |
Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol | acceptor XLLG-ol, recombinant TCH4 protein | Arabidopsis thaliana | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | TCH4 protein is not activated by Ca+ or other divalent cations | Arabidopsis thaliana |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| donor xyloglucan + xyloglucan acceptor | Arabidopsis thaliana | enzyme is unlikely to play a role in acid-induced wall loosening but may function in cold acclimation or cold-tolerant growth, TCH4 expression is rapidly regulated by mechanical stimuli, temperature shifts, light and hormones, it may be involved in assembling nascent cell walls or reinforcing existing and expanding walls | ? | - |
? | |
| donor xyloglucan + xyloglucan acceptor | Arabidopsis thaliana | existence of different classes of XET with differing roles in vivo | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
TCH4, member of the XET-related gene family | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | mature TCH4 polypeptide lacks the putative N-terminal signal sequence | Arabidopsis thaliana |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant TCH4 protein expressed in Escherichia coli | Arabidopsis thaliana |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| shoot | 26 days old | Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| donor xyloglucan + acceptor xyloglucan | xyloglucan from Tamarindus indica | Arabidopsis thaliana | ? | - |
? | |
| donor xyloglucan + acceptor xyloglucan | donors: TCH4 protein is more active against tamarind xyloglucan than nasturtium xyloglucan as donor, pea stem xyloglucan, acceptors: XLLG-ol, XXFG-ol | Arabidopsis thaliana | ? | - |
? | |
| donor xyloglucan + acceptor xyloglucan | xyloglucan from Tropaeolum majus seed | Arabidopsis thaliana | ? | - |
? | |
| donor xyloglucan + acceptor xyloglucan | donor specificity | Arabidopsis thaliana | ? | - |
? | |
| donor xyloglucan + acceptor xyloglucan | specific for xyloglucan | Arabidopsis thaliana | ? | - |
? | |
| donor xyloglucan + acceptor xyloglucan | TCH4 protein specifically transglycosylates only xyloglucan, cleavage of both fucosylated and nonfucosylated xyloglucans as donor substrates, but fucosyl content of the substrates lowers reaction rate, preferred acceptor substrate is the nonreducing terminus of high-MW xyloglucan, xyloglucan derived oligosaccharides are also utilized | Arabidopsis thaliana | ? | - |
? | |
| donor xyloglucan + Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)(Fuc-Gal-Xyl(1-6))Glc(1-4)Glc | - |
Arabidopsis thaliana | ? | - |
? | |
| donor xyloglucan + Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)(Fuc-Gal-Xyl(1-6))Glc(1-4)Glc-ol | XXFG-ol | Arabidopsis thaliana | ? | - |
? | |
| donor xyloglucan + Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)(Fuc-Gal-Xyl(1-6))Glc(1-4)Glc-ol | TCH4 protein, 50% as effective as XLLG-ol | Arabidopsis thaliana | ? | - |
? | |
| donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol | TCH4 protein, better substrate than XXFG-ol, lower affinity than for polymer xyloglucan | Arabidopsis thaliana | ? | - |
? | |
| donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol | XLLG-ol | Arabidopsis thaliana | ? | - |
? | |
| donor xyloglucan + xyloglucan acceptor | enzyme is unlikely to play a role in acid-induced wall loosening but may function in cold acclimation or cold-tolerant growth, TCH4 expression is rapidly regulated by mechanical stimuli, temperature shifts, light and hormones, it may be involved in assembling nascent cell walls or reinforcing existing and expanding walls | Arabidopsis thaliana | ? | - |
? | |
| donor xyloglucan + xyloglucan acceptor | existence of different classes of XET with differing roles in vivo | Arabidopsis thaliana | ? | - |
? | |
| additional information | not: carboxymethylcellulose, barley glucan | Arabidopsis thaliana | ? | - |
? | |
| pea xyloglucan | - |
Arabidopsis thaliana | ? | - |
? | |
| tamarind xyloglucan | - |
Arabidopsis thaliana | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 12 | 18 | recombinant TCH4 protein | Arabidopsis thaliana |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 18 | 26 | recombinant TCH4 protein, more active at lower temperatures, at or below 18°C, less active at higher temperatures, above 26°C | Arabidopsis thaliana |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 44 | - |
TCH4 protein: undetectable activity, more heat-sensitive than the total XET activity | Arabidopsis thaliana |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00018 | - |
tamarind xyloglucan | recombinant TCH4 protein, with XLLG-ol as acceptor | Arabidopsis thaliana | |
| 0.00031 | - |
pea xyloglucan | recombinant TCH4 protein, with XLLG-ol as acceptor | Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 6.5 | - |
Arabidopsis thaliana |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7.5 | pH 5: about 30% of maximal activity, pH 7.5: about 40% of maximal activity | Arabidopsis thaliana |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
