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Literature summary for 2.4.1.207 extracted from

  • Arrowsmith, D.A.; de Silva, J.
    Characterisation of two tomato fruit-expressed cDNAs encoding xyloglucan endo-transglycosylase (1995), Plant Mol. Biol., 28, 391-403.
    View publication on PubMed
Search for more literature for 2.4.1.207

Cloned(Commentary)

Cloned (Comment) Organism
small multi-gene family encodes XET: tXET–B1, -B2, -B3 and -B4, tXET-B2 is cloned, sequenced and overexpressed in Escherichia coli, nucleotide and amino acid sequence of tXET-B1 Solanum lycopersicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30460
-
 x * 30460, tXET-B2 Solanum lycopersicum
30493
-
 x * 30493, tXET-B1 Solanum lycopersicum
32000
-
 x * 32000, His-tagged recombinant tXET-B2 with predicted molecular mass of 32 kDa Solanum lycopersicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
donor xyloglucan + xyloglucan acceptor Solanum lycopersicum important component of cell wall metabolism, particularly expanding tissue and ripening fruits, study on the role in tomato fruit ripening and vegetative growth, tXET-B1 may incorporate new xyloglucan into the existing network ?
-
 ?

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum
-
 cv. Murrieta
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein tXET-B1 and tXET-B2 with N-glycosylation sites Solanum lycopersicum
proteolytic modification tXET-B1 and tXET-B2 contain a hydrophobic signal peptide of 20 and 18 amino acids, respectively, typical of an exported protein Solanum lycopersicum

Purification (Commentary)

Purification (Comment) Organism
His-tagged recombinant tXET-B2, expressed in Escherichia coli Solanum lycopersicum

Source Tissue

Source Tissue Comment Organism Textmining
fruit tXET-B1 is most abundant in pink fruit pericarp Solanum lycopersicum
-
stem tXET-B1 is detected in stems Solanum lycopersicum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
donor xyloglucan + acceptor xyloglucan acceptors: polymer xyloglucan or its derived oligosaccharides Solanum lycopersicum ?
-
 ?
donor xyloglucan + xyloglucan acceptor important component of cell wall metabolism, particularly expanding tissue and ripening fruits, study on the role in tomato fruit ripening and vegetative growth, tXET-B1 may incorporate new xyloglucan into the existing network Solanum lycopersicum ?
-
 ?
additional information tXET-B2 has no hydrolytic activity Solanum lycopersicum ?
-
 ?

Subunits

Subunits Comment Organism
? x * 30493, tXET-B1 Solanum lycopersicum
? x * 32000, His-tagged recombinant tXET-B2 with predicted molecular mass of 32 kDa Solanum lycopersicum
? x * 30460, tXET-B2 Solanum lycopersicum