Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.207 extracted from

  • Sulova, Z.; Lednicka, M.; Farkas, V.
    A colorimetric assay for xyloglucan-endotransglycosylase from germinating seeds (1995), Anal. Biochem., 229, 80-85.
    View publication on PubMed
Search for more literature for 2.4.1.207

Activating Compound

Activating Compound Comment Organism Structure
xyloglucan-derived oligosaccharide stimulates, highest stimulating effect with the nonasaccharide Glc4Xyl3Gal2, lowest one with the pentasaccharide Glc3Xyl2 Tropaeolum majus

General Stability

General Stability Organism
0.1 mM final concentration spermine stabilizes Tropaeolum majus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
donor xyloglucan + xyloglucan acceptor Phaseolus vulgaris one of the key enzymes involved in the breakdown of reserve xyloglucan in seeds of some dicotyledonous plants during germination ?
-
 ?
donor xyloglucan + xyloglucan acceptor Pisum sativum one of the key enzymes involved in the breakdown of reserve xyloglucan in seeds of some dicotyledonous plants during germination ?
-
 ?
donor xyloglucan + xyloglucan acceptor Lens culinaris one of the key enzymes involved in the breakdown of reserve xyloglucan in seeds of some dicotyledonous plants during germination ?
-
 ?
donor xyloglucan + xyloglucan acceptor Tamarindus indica one of the key enzymes involved in the breakdown of reserve xyloglucan in seeds of some dicotyledonous plants during germination ?
-
 ?
donor xyloglucan + xyloglucan acceptor Tropaeolum majus one of the key enzymes involved in the breakdown of reserve xyloglucan in seeds of some dicotyledonous plants during germination ?
-
 ?

Organism

Organism UniProt Comment Textmining
Lens culinaris
-
-
-
Phaseolus vulgaris
-
-
-
Pisum sativum
-
-
-
Tamarindus indica
-
-
-
Tropaeolum majus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Phaseolus vulgaris
-
 Pisum sativum
-
 Lens culinaris
-
 Tamarindus indica
-
 Tropaeolum majus

Reaction

Reaction Comment Organism Reaction ID
2 Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc = Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glc + Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc transglycosylic mechanism, i.e. random splitting of the beta-1,4-linked polyglucose backbone of xyloglucan molecules and rejoining the newly created reducing ends by beta-1,4 glycosidic bonds to nonreducing ends of other xyloglucan molecules or xyloglucan subunit oligosaccharides Phaseolus vulgaris
a
2 Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc = Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glc + Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc transglycosylic mechanism, i.e. random splitting of the beta-1,4-linked polyglucose backbone of xyloglucan molecules and rejoining the newly created reducing ends by beta-1,4 glycosidic bonds to nonreducing ends of other xyloglucan molecules or xyloglucan subunit oligosaccharides Pisum sativum
a
2 Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc = Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glc + Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc transglycosylic mechanism, i.e. random splitting of the beta-1,4-linked polyglucose backbone of xyloglucan molecules and rejoining the newly created reducing ends by beta-1,4 glycosidic bonds to nonreducing ends of other xyloglucan molecules or xyloglucan subunit oligosaccharides Lens culinaris
a
2 Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc = Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glc + Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc transglycosylic mechanism, i.e. random splitting of the beta-1,4-linked polyglucose backbone of xyloglucan molecules and rejoining the newly created reducing ends by beta-1,4 glycosidic bonds to nonreducing ends of other xyloglucan molecules or xyloglucan subunit oligosaccharides Tamarindus indica
a
2 Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc = Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glc + Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc transglycosylic mechanism, i.e. random splitting of the beta-1,4-linked polyglucose backbone of xyloglucan molecules and rejoining the newly created reducing ends by beta-1,4 glycosidic bonds to nonreducing ends of other xyloglucan molecules or xyloglucan subunit oligosaccharides Tropaeolum majus
a

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon of germinated seeds Phaseolus vulgaris
-
cotyledon of germinated seeds Pisum sativum
-
cotyledon of germinated seeds Lens culinaris
-
cotyledon of germinated seeds Tamarindus indica
-
cotyledon of germinated seeds Tropaeolum majus
-
seed cotyledons of germinated seeds Phaseolus vulgaris
-
seed cotyledons of germinated seeds Pisum sativum
-
seed cotyledons of germinated seeds Lens culinaris
-
seed cotyledons of germinated seeds Tamarindus indica
-
seed cotyledons of germinated seeds Tropaeolum majus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 low cost, simple, high-speed colorimetric assay that allows to analyze multiple samples simultaneously Phaseolus vulgaris
additional information
-
 low cost, simple, high-speed colorimetric assay that allows to analyze multiple samples simultaneously Pisum sativum
additional information
-
 low cost, simple, high-speed colorimetric assay that allows to analyze multiple samples simultaneously Lens culinaris
additional information
-
 low cost, simple, high-speed colorimetric assay that allows to analyze multiple samples simultaneously Tamarindus indica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
donor xyloglucan + acceptor xyloglucan acceptors: polymer xyloglucan or its derived oligosaccharides Phaseolus vulgaris ?
-
 ?
donor xyloglucan + acceptor xyloglucan acceptors: polymer xyloglucan or its derived oligosaccharides Pisum sativum ?
-
 ?
donor xyloglucan + acceptor xyloglucan acceptors: polymer xyloglucan or its derived oligosaccharides Lens culinaris ?
-
 ?
donor xyloglucan + acceptor xyloglucan acceptors: polymer xyloglucan or its derived oligosaccharides Tamarindus indica ?
-
 ?
donor xyloglucan + acceptor xyloglucan acceptors: polymer xyloglucan or its derived oligosaccharides Tropaeolum majus ?
-
 ?
donor xyloglucan + acceptor xyloglucan acceptor: fucosylated xyloglucan nonasaccharide Tropaeolum majus ?
-
 ?
donor xyloglucan + acceptor xyloglucan xyloglucan from Tamarindus indica Phaseolus vulgaris ?
-
 ?
donor xyloglucan + acceptor xyloglucan xyloglucan from Tamarindus indica Pisum sativum ?
-
 ?
donor xyloglucan + acceptor xyloglucan xyloglucan from Tamarindus indica Lens culinaris ?
-
 ?
donor xyloglucan + acceptor xyloglucan xyloglucan from Tamarindus indica Tamarindus indica ?
-
 ?
donor xyloglucan + acceptor xyloglucan xyloglucan from Tamarindus indica Tropaeolum majus ?
-
 ?
donor xyloglucan + Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)Glc
-
 Phaseolus vulgaris ?
-
 ?
donor xyloglucan + Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)Glc
-
 Pisum sativum ?
-
 ?
donor xyloglucan + Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)Glc Glc3Xyl2 Tropaeolum majus ?
-
 ?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc
-
 Pisum sativum ?
-
 ?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc Glc4Xyl3Gal2 Tropaeolum majus ?
-
 ?
donor xyloglucan + xyloglucan acceptor one of the key enzymes involved in the breakdown of reserve xyloglucan in seeds of some dicotyledonous plants during germination Phaseolus vulgaris ?
-
 ?
donor xyloglucan + xyloglucan acceptor one of the key enzymes involved in the breakdown of reserve xyloglucan in seeds of some dicotyledonous plants during germination Pisum sativum ?
-
 ?
donor xyloglucan + xyloglucan acceptor one of the key enzymes involved in the breakdown of reserve xyloglucan in seeds of some dicotyledonous plants during germination Lens culinaris ?
-
 ?
donor xyloglucan + xyloglucan acceptor one of the key enzymes involved in the breakdown of reserve xyloglucan in seeds of some dicotyledonous plants during germination Tamarindus indica ?
-
 ?
donor xyloglucan + xyloglucan acceptor one of the key enzymes involved in the breakdown of reserve xyloglucan in seeds of some dicotyledonous plants during germination Tropaeolum majus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Phaseolus vulgaris
37
-
 assay at Pisum sativum
37
-
 assay at Lens culinaris
37
-
 assay at Tamarindus indica
37
-
 assay at Tropaeolum majus