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Literature summary for 2.4.1.207 extracted from

  • Bourquin, V.; Nishikubo, N.; Abe, H.; Brumer, H.; Denman, S.; Eklund, M.; Christiernin, M.; Teeri, T.T.; Sundberg, B.; Mellerowicz, E.J.
    Xyloglucan endotransglycosylases have a function during the formation of secondary cell walls of vascular tissues (2002), Plant Cell, 14, 3073-3088.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
PttXET16A, a XET isoform in secondary vascular tissues and a member of XET subfamily I, is cloned, sequenced and expressed in Escherichia coli BL21(DE3), structure of the coding sequence Populus tremula x Populus tremuloides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
donor xyloglucan + xyloglucan acceptor Populus tremula x Populus tremuloides multifunctional role in cell wall construction, role in restructuring primary cell walls at the time when secondary wall layers are deposited, probably creating and reinforcing the connections between the primary and secondary wall layers ?
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?

Organism

Organism UniProt Comment Textmining
Populus tremula x Populus tremuloides Q8GZD5 hybrid aspen, poplar, major isoenzyme PttXET16A, a member of XET subfamily I
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Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein PttXET16A contains a putative N-glycosylation site Populus tremula x Populus tremuloides
proteolytic modification PttXET16A has a putative 22 amino acids signal peptide at the N-terminus Populus tremula x Populus tremuloides

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged PttXET16A, expressed in Escherichia coli BL21(DE3) Populus tremula x Populus tremuloides

Source Tissue

Source Tissue Comment Organism Textmining
leaf PttXET16A is expressed transiently in developing leaves Populus tremula x Populus tremuloides
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additional information detailed localization of XET in poplar stems, PttXET16A expression pattern, expression in secondary vascular tissues Populus tremula x Populus tremuloides
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phloem XET activity in xylem and phloem fibers at the stage of secondary wall formation, PttXET16A Populus tremula x Populus tremuloides
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root PttXET16A expression in young roots and root tips Populus tremula x Populus tremuloides
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stem
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Populus tremula x Populus tremuloides
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xylem XET activity in xylem and phloem fibers at the stage of secondary wall formation, PttXET16A Populus tremula x Populus tremuloides
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
donor xyloglucan + acceptor xyloglucan acceptors: sulforhodamine-labeled xyloglucan-oligosaccharides Populus tremula x Populus tremuloides ?
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?
donor xyloglucan + xyloglucan acceptor multifunctional role in cell wall construction, role in restructuring primary cell walls at the time when secondary wall layers are deposited, probably creating and reinforcing the connections between the primary and secondary wall layers Populus tremula x Populus tremuloides ?
-
?
additional information contains strongly conserved sequence motif of XETs: DEIDFEFLG Populus tremula x Populus tremuloides ?
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?
additional information PttXET16A has 4 conserved Cys residues Populus tremula x Populus tremuloides ?
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?