Cloned (Comment) | Organism |
---|---|
PttXET16A, a XET isoform in secondary vascular tissues and a member of XET subfamily I, is cloned, sequenced and expressed in Escherichia coli BL21(DE3), structure of the coding sequence | Populus tremula x Populus tremuloides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
donor xyloglucan + xyloglucan acceptor | Populus tremula x Populus tremuloides | multifunctional role in cell wall construction, role in restructuring primary cell walls at the time when secondary wall layers are deposited, probably creating and reinforcing the connections between the primary and secondary wall layers | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Populus tremula x Populus tremuloides | Q8GZD5 | hybrid aspen, poplar, major isoenzyme PttXET16A, a member of XET subfamily I | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | PttXET16A contains a putative N-glycosylation site | Populus tremula x Populus tremuloides |
proteolytic modification | PttXET16A has a putative 22 amino acids signal peptide at the N-terminus | Populus tremula x Populus tremuloides |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged PttXET16A, expressed in Escherichia coli BL21(DE3) | Populus tremula x Populus tremuloides |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | PttXET16A is expressed transiently in developing leaves | Populus tremula x Populus tremuloides | - |
additional information | detailed localization of XET in poplar stems, PttXET16A expression pattern, expression in secondary vascular tissues | Populus tremula x Populus tremuloides | - |
phloem | XET activity in xylem and phloem fibers at the stage of secondary wall formation, PttXET16A | Populus tremula x Populus tremuloides | - |
root | PttXET16A expression in young roots and root tips | Populus tremula x Populus tremuloides | - |
stem | - |
Populus tremula x Populus tremuloides | - |
xylem | XET activity in xylem and phloem fibers at the stage of secondary wall formation, PttXET16A | Populus tremula x Populus tremuloides | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
donor xyloglucan + acceptor xyloglucan | acceptors: sulforhodamine-labeled xyloglucan-oligosaccharides | Populus tremula x Populus tremuloides | ? | - |
? | |
donor xyloglucan + xyloglucan acceptor | multifunctional role in cell wall construction, role in restructuring primary cell walls at the time when secondary wall layers are deposited, probably creating and reinforcing the connections between the primary and secondary wall layers | Populus tremula x Populus tremuloides | ? | - |
? | |
additional information | contains strongly conserved sequence motif of XETs: DEIDFEFLG | Populus tremula x Populus tremuloides | ? | - |
? | |
additional information | PttXET16A has 4 conserved Cys residues | Populus tremula x Populus tremuloides | ? | - |
? |