Cloned (Comment) | Organism |
---|---|
phylogenetic tree and expression analysis of XTH genes, transgenic overexpression of PtxtXET16-34 in Populus tremula x tremuloides | Populus tremula x Populus tremuloides |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | - |
Populus tremula x Populus tremuloides | 5618 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Populus tremula x Populus tremuloides | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
xylem | developing, at least 16 Populus XTH genes are expressed in developing wood. Five genes are highly and ubiquitously expressed, whereas PtxtXET16-34 is expressed more weakly but specifically in developing wood | Populus tremula x Populus tremuloides | - |
xylem | primary- and secondary-walled xylem tissues | Populus tremula x Populus tremuloides | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | activity assay with reduced xyloglucan-derived nonasaccharide as substrate | Populus tremula x Populus tremuloides | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
XET | - |
Populus tremula x Populus tremuloides |
XTH | - |
Populus tremula x Populus tremuloides |
xyloglucan endo-transglycosylase | - |
Populus tremula x Populus tremuloides |
xyloglucan endo-transglycosylases/hydrolase | - |
Populus tremula x Populus tremuloides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Populus tremula x Populus tremuloides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
assay at | Populus tremula x Populus tremuloides |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the GH16 XTH family, gene models, overview | Populus tremula x Populus tremuloides |
additional information | transgenic up-regulation of XET activity induced changes in cell wall xyloglucan, but its effects are dependent on developmental stage. For instance, XET overexpression increases abundance of the CCRC-M1 epitope in cambial cells and xylem cells in early stages of differentiation but not in mature xylem. Correspondingly, an increase in tightly bound xyloglucan content is observed in primary-walled xylem but a decrease is seen in secondary-walled xylem | Populus tremula x Populus tremuloides |
physiological function | xyloglucan endo-transglycosylases, XETs, encoded by xyloglucan endo-transglycosylases/hydrolase, XTH, genes modify the xyloglucan-cellulose framework of plant cell walls, thereby regulating their expansion and strength. Importance of XET in wood development, xyloglucan dynamics and XTH gene expression in developing wood, overview. Several XTH genes are involved in the development of secondary vascular tissues, XET activity stimulates cell expansion in vessel elements but not in fibers. In young xylem cells, XET activity limits xyloglucan incorporation into the tightly bound wall network but removes it from cell walls in older cells. Importance of XET activity for vascular tissue differentiation and/or function. Increased XET activity has differing effects on the xyloglucan content of expanding and maturing wood cells | Populus tremula x Populus tremuloides |