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Literature summary for 2.4.1.21 extracted from
- The purification and characterization of the two forms of soluble starch synthase from developing pea embryos (1992), Planta, 186, 609-617.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | amylopectin: 0.19-0.79 mg/ml, value depends on isozyme and presence of citrate | Pisum sativum | |
| 0.29 | - |
ADPglucose | synthase I, absence of citrate | Pisum sativum |  |
| 0.48 | - |
ADPglucose | synthase II, absence of citrate | Pisum sativum | |
| 0.51 | - |
ADPglucose | synthase II, presence of citrate | Pisum sativum | |
| 0.97 | - |
ADPglucose | synthase I, presence of citrate | Pisum sativum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Pisum sativum | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
x * 60000, synthase I, x * 77000, synthase II, SDS-PAGE | Pisum sativum |
| 77000 | - |
x * 60000, synthase I, x * 77000, synthase II, SDS-PAGE | Pisum sativum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pisum sativum | - |
pea | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 2 isoforms, DEAE-Sepharose, ammonium sulfate, Blue Sepharose, omega-aminobutyl agarose, Mono Q | Pisum sativum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Pisum sativum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 15.87 | - |
- |
Pisum sativum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP-glucose + alpha-1,4-polyglucan | no activity with UDPglucose | Pisum sativum | ADP + alpha-1,4-polyglucan | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 60000, synthase I, x * 77000, synthase II, SDS-PAGE | Pisum sativum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | - |
stable at | Pisum sativum |
| 25 | - |
stable at | Pisum sativum |
| 35 | - |
inactivation | Pisum sativum |
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Release 2023.1 (January 2023)
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