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Literature summary for 2.4.1.21 extracted from

  • Frydman, R.B.; Cardini, C.E.
    Studies on adenosine diphosphate D-glucose:alpha-(1-4)-glucan alpha4-glucosyltransferase of sweet-corn endosperm (1965), Biochim. Biophys. Acta, 96, 294-303.
    View publication on PubMed
Search for more literature for 2.4.1.21

Inhibitors

Inhibitors Comment Organism Structure
adenosine 0.7 mM, 30% inhibition Zea mays subsp. mays
ADP 0.7 mM, 30% inhibition Zea mays subsp. mays
Ammonium molybdate 7 mM, complete inhibition Zea mays subsp. mays
AMP 0.7 mM, 30% inhibition Zea mays subsp. mays
ATP
-
 Zea mays subsp. mays
CaCl2 0.8 mM, 35% inhibition Zea mays subsp. mays
CoCl2 4 mM, 70% inhibition Zea mays subsp. mays
dADPglucose 1 mM, 30% inhibition Zea mays subsp. mays
HgCl2 0.3 mM, complete inhibition Zea mays subsp. mays
hydroquinone 5 mM, complete inhibition Zea mays subsp. mays
MgCl2
-
 Zea mays subsp. mays
MnCl2 7 mM, 30% inhibition Zea mays subsp. mays
p-chloromercuribenzoate 7 mM, complete inhibition Zea mays subsp. mays
ZnSO4 7 mM, complete inhibition Zea mays subsp. mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Zea mays subsp. mays
0.75
-
 amylopectin
-
 Zea mays subsp. mays
1.1
-
 phytoglycogen
-
 Zea mays subsp. mays
16.6
-
 maltotriose
-
 Zea mays subsp. mays

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Zea mays subsp. mays
-
-

Organism

Organism UniProt Comment Textmining
Zea mays subsp. mays
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, DEAE-cellulose Zea mays subsp. mays

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Zea mays subsp. mays
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
65.47
-
-
 Zea mays subsp. mays

Storage Stability

Storage Stability Organism
-10°C, fraction V, unstable Zea mays subsp. mays
-15°C, fraction IV, 6 months Zea mays subsp. mays

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP-alpha-D-glucose + maltotriose
-
 Zea mays subsp. mays ADP + maltotetraose
-
 ?
ADP-alpha-D-glucose + phytoglycogen
-
 Zea mays subsp. mays ADP + ?
-
 ?
ADP-glucose + alpha-1,4-polyglucan glucosyl acceptor: starch Zea mays subsp. mays ADP + alpha-1,4-polyglucan
-
 ?
ADP-glucose + alpha-1,4-polyglucan glucosyl acceptors: phytoglycogen, maltotriose, maltotetraose or maltopentaose Zea mays subsp. mays ADP + alpha-1,4-polyglucan
-
 ?
ADP-glucose + alpha-1,4-polyglucan glucosyl acceptor: amylopectin Zea mays subsp. mays ADP + alpha-1,4-polyglucan
-
 ?
ADP-glucose + amylopectin
-
 Zea mays subsp. mays ADP + ?
-
 ?
dADPglucose + alpha-1,4-polyglucan 8% of activity with ADPglucose Zea mays subsp. mays dADP + alpha-1,4-polyglucan
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
24 30 65% activity at 37°C Zea mays subsp. mays

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 10 min, 78% loss of activity Zea mays subsp. mays
50
-
 1 min, 75% loss of activity Zea mays subsp. mays
62
-
 inactivation at Zea mays subsp. mays

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3
-
-
 Zea mays subsp. mays

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9.7 less than half-maximal activity above and below Zea mays subsp. mays