Cloned (Comment) | Organism |
---|---|
- |
Zea mays |
Protein Variants | Comment | Organism |
---|---|---|
K193E | mutation does not alter the binding of ADP-glucose, Km-value for amylopectin is 3.6fold higher than the wild-type value, KM-value for glycogen is 3.2fold lower than the wild-type value | Zea mays |
K193Q | mutation does not alter the binding of ADP-glucose, Km-value for amylopectin is 1.5fold lower than the wild-type value, KM-value for glycogen is 15.5fold lower than the wild-type value | Zea mays |
K193R | mutation does not alter the binding of ADP-glucose, Km-value for amylopectin is 2.6fold higher than the wild-type value, KM-value for glycogen is 2.5fold lower than the wild-type value | Zea mays |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
pyridoxal-5'-phosphate | inactivates starch synthase IIa, ADP-glucose protects from inactivation | Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km-values for amylopectin: 0.2 mg/ml for wild-type enzyme, 0.53 mg/ml for mutant enzyme K193R, 0.13 mg/ml for mutant enzyme K193Q, 0.72 mg/ml for mutant enzyme K193E. Km-values for glycogen: 0.17 mg/ml for wild-type enzyme, 0.68 mg/ml for mutant enzyme K193R, 0.11 mg/ml for mutant enzyme K193Q, 0.53 mg/ml for mutant enzyme K193E | Zea mays | |
0.1 | - |
ADP-glucose | mutant enzyme K193Q, glycogen as primer | Zea mays | |
0.11 | - |
ADP-glucose | wild-type enzyme, amylopectin as primer | Zea mays | |
0.13 | - |
ADP-glucose | wild-type enzyme, glycogen as primer | Zea mays | |
0.14 | - |
ADP-glucose | mutant enzyme K193Q, amylopectin as primer | Zea mays | |
0.15 | - |
ADP-glucose | mutant enzyme K193R, amylopectin as primer | Zea mays | |
0.15 | - |
ADP-glucose | mutant enzyme K193R, glycogen as primer | Zea mays | |
0.17 | - |
ADP-glucose | mutant enzyme K193E, glycogen as primer | Zea mays | |
0.22 | - |
ADP-glucose | mutant enzyme K193E, amylopectin as primer | Zea mays |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and mutant enzymes expressed in Escherichia coli | Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-glucose + amylopectin | - |
Zea mays | ADP + ? | - |
? | |
ADP-glucose + glycogen | - |
Zea mays | ADP + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
starch synthase IIa | - |
Zea mays |