Literature summary for 2.4.1.21 extracted from

Sheng, F.; Jia, X.; Yep, A.; Preiss, J.; Geiger, J.H.
The crystal structures of the open and catalytically competent closed conformation of Escherichia coli glycogen synthase (2009), J. Biol. Chem., 284, 17796-17807.

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
structure of the wild-type enzyme bound to ADP and glucose reveals a 15.2° overall domain-domain closure. The main chain carbonyl group of His-161, Arg-300, and Lys-305 are suggested to act as critical catalytic residues in the transglycosylation. Glu-377 is found on the-face of the glucose and plays an electrostatic role in the active site and as a glucose ring locator. In the mutant E377A-ADP-4-(2-hydroxyethyl)piperazine-1-(2-hydroxypropane)sulfonic acid complex the glucose moiety is either absent or disordered in the active site	Escherichia coli

Crystallization (Comment)

Organism

structure of the wild-type enzyme bound to ADP and glucose reveals a 15.2° overall domain-domain closure. The main chain carbonyl group of His-161, Arg-300, and Lys-305 are suggested to act as critical catalytic residues in the transglycosylation. Glu-377 is found on the-face of the glucose and plays an electrostatic role in the active site and as a glucose ring locator. In the mutant E377A-ADP-4-(2-hydroxyethyl)piperazine-1-(2-hydroxypropane)sulfonic acid complex the glucose moiety is either absent or disordered in the active site

Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
C7S/C409S	exhibits comparable specific activity and apparent affinity for ADP-Glc to wild-type. Use as alternative in crystallization trials to avoid aggregation	Escherichia coli
E377A	crystallization data, in the mutant E377A-ADP-4-(2-hydroxyethyl)piperazine-1-(2-hydroxypropane)sulfonic acid complex the glucose moiety is either absent or disordered in the active site	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P0A6U8	-	-

Synonyms

Synonyms	Comment	Organism
glycogen synthase	-	Escherichia coli

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)