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Literature summary for 2.4.1.21 extracted from

  • Skryhan, K.; Cuesta-Seijo, J.A.; Nielsen, M.M.; Marri, L.; Mellor, S.B.; Glaring, M.A.; Jensen, P.E.; Palcic, M.M.; Blennow, A.
    The role of cysteine residues in redox regulation and protein stability of Arabidopsis thaliana starch synthase 1 (2015), PLoS ONE, 10, e0136997.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.21

Activating Compound

Activating Compound Comment Organism Structure
reduced thioredoxin f1
-
 Arabidopsis thaliana
reduced thioredoxin m4
-
 Arabidopsis thaliana
thioredoxin
-
 Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
C164S the mutant shows reduced activity compared to the wild type enzyme Arabidopsis thaliana
C164S the mutant shows 50% activity compared to the wild type enzyme Arabidopsis thaliana
C164S/C265S the mutant shows strongly reduced activity compared to the wild type enzyme Arabidopsis thaliana
C164S/C545S the mutant shows strongly reduced activity compared to the wild type enzyme Arabidopsis thaliana
C164S/C545S the double mutant with 13% of wild type activity has considerably decreased redox sensitivity as compared to wild type enzyme (30% vs 77%) Arabidopsis thaliana
C209S the mutant shows wild type activity Arabidopsis thaliana
C261S the mutant shows reduced activity compared to the wild type enzyme Arabidopsis thaliana
C265S the mutant shows reduced activity compared to the wild type enzyme Arabidopsis thaliana
C265S the mutant shows 37% activity compared to the wild type enzyme Arabidopsis thaliana
C265S/C545S the mutant shows strongly reduced activity compared to the wild type enzyme Arabidopsis thaliana
C265S/C545S the mutant shows 8% activity compared to the wild type enzyme Arabidopsis thaliana
C442S the mutant shows reduced activity compared to the wild type enzyme Arabidopsis thaliana
C458S the mutant shows reduced activity compared to the wild type enzyme Arabidopsis thaliana
C533S the mutant shows wild type activity Arabidopsis thaliana
C545S the mutant shows 27% activity compared to the wild type enzyme Arabidopsis thaliana
C545S the mutant shows strongly reduced activity compared to the wild type enzyme Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Arabidopsis thaliana
oxidized thioredoxin f1
-
 Arabidopsis thaliana
oxidized thioredoxin m4
-
 Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Arabidopsis thaliana Q9FNF2
-
-

Purification (Commentary)

Purification (Comment) Organism
His-Trap column chromatography Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP-alpha-D-glucose + glycogen 187% activity compared to maltotriose Arabidopsis thaliana ADP + ?
-
 ?
ADP-alpha-D-glucose + maltoheptaose 80% activity compared to maltotriose Arabidopsis thaliana ADP + maltooctaose
-
 ?
ADP-alpha-D-glucose + maltohexaose 90% activity compared to maltotriose Arabidopsis thaliana ADP + maltoheptaose
-
 ?
ADP-alpha-D-glucose + maltooctaose 79% activity compared to maltotriose Arabidopsis thaliana ADP + maltononaose
-
 ?
ADP-alpha-D-glucose + maltopentaose 116% activity compared to maltotriose Arabidopsis thaliana ADP + maltohexaose
-
 ?
ADP-alpha-D-glucose + maltose 20% activity compared to maltotriose Arabidopsis thaliana ADP + ?
-
 ?
ADP-alpha-D-glucose + maltose 20% activity compared to maltotriose Arabidopsis thaliana ADP + maltotriose
-
 ?
ADP-alpha-D-glucose + maltotetraose 103% activity compared to maltotriose Arabidopsis thaliana ADP + maltopentaose
-
 ?
ADP-alpha-D-glucose + maltotriose 100% activity Arabidopsis thaliana ADP + maltotetraose
-
 ?
additional information less than 1% activity with D-glucose Arabidopsis thaliana ?
-
 ?

Synonyms

Synonyms Comment Organism
SS1
-
 Arabidopsis thaliana
starch synthase 1
-
 Arabidopsis thaliana

Expression

Organism Comment Expression
Arabidopsis thaliana the enzyme is in the reduced and active form during the day with active photosynthesis up