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Literature summary for 2.4.1.210 extracted from
- Purification of limonoid glucosyltransferase from navel orange albedo tissue (1997), Phytochemistry, 46, 33-37.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| nutrition | limonoid glucosides are important compounds not only for the processing industry but also for the consumer, bitterness due to limonoids is an important economic problem in commercial citrus juice production, limonoid aglycones are converted to nonbitter glucosides by the GTase, enhancement of the limonoid GTase activity through genetic engineering could reduce aglycone concentration, insertion of a gene encoding for GTase into commercial cultivars could create transgenic citrus varieties producing fruits potentially free of limonoid bitterness | Citrus sinensis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| PO43- | - |
Fragaria vesca |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | 0.05 mM stimulates enzyme activity by 66% over basal activity | Citrus sinensis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
Newhall navel orange, SDS-PAGE | Citrus sinensis |
| 58000 | - |
Frost navel orange, SDS-PAGE | Citrus sinensis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| limonin + UDP-glucose | Citrus sinensis | - |
glucosyl-limonin + UDP | - |
? | |
| limonin + UDP-glucose | Citrus unshiu | - |
glucosyl-limonin + UDP | - |
? | |
| limonin + UDP-glucose | Citrus aurantium | - |
glucosyl-limonin + UDP | - |
? | |
| limonin + UDP-glucose | Fragaria vesca | - |
glucosyl-limonin + UDP | - |
? | |
| limonin + UDP-glucose | Citrus maxima | - |
glucosyl-limonin + UDP | - |
? | |
| limonin + UDP-glucose | Citrus limonia | - |
glucosyl-limonin + UDP | - |
? | |
| nomilin + UDP-glucose | Citrus sinensis | - |
UDP + nomilin 17-beta-D-glucopyranoside | - |
? | |
| nomilin + UDP-glucose | Citrus unshiu | - |
UDP + nomilin 17-beta-D-glucopyranoside | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Citrus aurantium | - |
pommelo hybrid | - |
| Citrus limonia | - |
citron | - |
| Citrus maxima | - |
pommelo | - |
| Citrus maxima | - |
grapefruit, pommelo hybrid Oroblanco | - |
| Citrus sinensis | - |
Frost navel orange, Newhall navel orange | - |
| Citrus unshiu | - |
mandarin orange | - |
| Fragaria vesca | - |
strawberry | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Citrus sinensis |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| UDP-glucose + limonin + H2O = glucosyl-limonin + UDP | the enzyme purified from navel orange albedo tissue also acts on the related tetranortriterpenoid nomilin | Citrus sinensis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| albedo | - |
Citrus sinensis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.018 | - |
- |
Citrus sinensis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, 50 mM Tris-HCl, pH 8.0, all acivity is lost | Citrus sinensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| limonin + UDP-glucose | - |
Citrus sinensis | glucosyl-limonin + UDP | - |
? | |
| limonin + UDP-glucose | - |
Citrus unshiu | glucosyl-limonin + UDP | - |
? | |
| limonin + UDP-glucose | - |
Citrus aurantium | glucosyl-limonin + UDP | - |
? | |
| limonin + UDP-glucose | - |
Fragaria vesca | glucosyl-limonin + UDP | - |
? | |
| limonin + UDP-glucose | - |
Citrus maxima | glucosyl-limonin + UDP | - |
? | |
| limonin + UDP-glucose | - |
Citrus limonia | glucosyl-limonin + UDP | - |
? | |
| nomilin + UDP-glucose | - |
Citrus sinensis | glucosyl-nomilin + UDP | - |
? | |
| nomilin + UDP-glucose | - |
Citrus sinensis | UDP + nomilin 17-beta-D-glucopyranoside | - |
? | |
| nomilin + UDP-glucose | - |
Citrus unshiu | UDP + nomilin 17-beta-D-glucopyranoside | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 56000-58000, SDS-PAGE | Citrus sinensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Citrus sinensis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9 | - |
Citrus sinensis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
activity below pH 6.5 is inhibited by a closed D-ring | Citrus sinensis |
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