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Literature summary for 2.4.1.210 extracted from
- Isolation and characterization of limonoid glucosyltransferase from pummelo albedo tissue (2002), Food Chem., 76, 431-436.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 10 mM, 13% inhibition | Citrus maxima |  |
| citraconic anhydride | 2 mM, 44% inhibition | Citrus maxima | |
| Cu2+ | 5 mM, 58% inhibition | Citrus maxima | |
| diethyl dicarbonate | 1 mM, 76% inhibition | Citrus maxima | |
| EDTA | 1 mM, complete inactivation | Citrus maxima | |
| Hg2+ | 1 mM, 33% inhibition | Citrus maxima | |
| Mg2+ | 10 mM, 22% inhibition | Citrus maxima | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.065 | - |
UDP-glucose | pH 7.8, 37°C | Citrus maxima | |
| 0.2 | - |
limonin | pH 7.8, 37°C | Citrus maxima | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 5 mM, 30% stimulation | Citrus maxima | |
| Mn2+ | 5 mM, 33% stimulation | Citrus maxima | |
| Zn2+ | 5 mM, 13% stimulation | Citrus maxima | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Citrus maxima | - |
Osbeck | - |
| Citrus maxima Osbeck | - |
Osbeck | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Citrus maxima |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fruit | albedo tissue | Citrus maxima | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.013 | - |
- |
Citrus maxima |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, pH 7.5, 10 mM Tris-HCl, enzyme retains 95% of the activity after 3 months, enzyme retains 73% of the activity after 9 months | Citrus maxima |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-glucose + limonin | - |
Citrus maxima | glucosyl-limonin + UDP | - |
? | |
| UDP-glucose + limonin | - |
Citrus maxima Osbeck | glucosyl-limonin + UDP | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Citrus maxima |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 60 | 20°C: about 40% of maximal activity, 60°C: about 40% of maximal activity | Citrus maxima |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
- |
Citrus maxima |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9.5 | pH 6.5: about 70% of maximal activity, pH 9.5: about 55% of maximal activity | Citrus maxima |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 9.5 | 4°C, 12 h, stable | Citrus maxima |
| 10.5 | - |
4°C, 12 h, 62% loss of activity | Citrus maxima |
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