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Literature summary for 2.4.1.211 extracted from

  • Nishimoto, M.; Kitaoka, M.
    Identification of the putative proton donor residue of lacto-N-biose phosphorylase (EC 2.4.1.211) (2007), Biosci. Biotechnol. Biochem., 71, 1587-1591.
    View publication on PubMed
Search for more literature for 2.4.1.211

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bifidobacterium bifidum JCM 1254

Protein Variants

Protein Variants Comment Organism
D108N site-directed mutagenesis Bifidobacterium bifidum JCM 1254
D160N site-directed mutagenesis Bifidobacterium bifidum JCM 1254
D182N site-directed mutagenesis Bifidobacterium bifidum JCM 1254
D232N site-directed mutagenesis Bifidobacterium bifidum JCM 1254
D260N site-directed mutagenesis Bifidobacterium bifidum JCM 1254
D280N site-directed mutagenesis Bifidobacterium bifidum JCM 1254
D29N site-directed mutagenesis Bifidobacterium bifidum JCM 1254
D313N site-directed mutagenesis Bifidobacterium bifidum JCM 1254
D331N site-directed mutagenesis Bifidobacterium bifidum JCM 1254
D344N site-directed mutagenesis Bifidobacterium bifidum JCM 1254
D348N site-directed mutagenesis Bifidobacterium bifidum JCM 1254
D35N site-directed mutagenesis Bifidobacterium bifidum JCM 1254
D373N site-directed mutagenesis Bifidobacterium bifidum JCM 1254
D490N site-directed mutagenesis Bifidobacterium bifidum JCM 1254
D491N site-directed mutagenesis Bifidobacterium bifidum JCM 1254
D561N site-directed mutagenesis Bifidobacterium bifidum JCM 1254
E249Q site-directed mutagenesis Bifidobacterium bifidum JCM 1254
E259Q site-directed mutagenesis Bifidobacterium bifidum JCM 1254
E319Q site-directed mutagenesis Bifidobacterium bifidum JCM 1254
E356Q site-directed mutagenesis Bifidobacterium bifidum JCM 1254
E377Q site-directed mutagenesis Bifidobacterium bifidum JCM 1254
E475Q site-directed mutagenesis Bifidobacterium bifidum JCM 1254
E568Q site-directed mutagenesis Bifidobacterium bifidum JCM 1254
E691Q site-directed mutagenesis Bifidobacterium bifidum JCM 1254

Organism

Organism UniProt Comment Textmining
Bifidobacterium bifidum JCM 1254
-
-
-

Purification (Commentary)

Purification (Comment) Organism
of the recombinant proteins by Ni-NTA spin column chromatography Bifidobacterium bifidum JCM 1254

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0002
-
 D313N, no activity, D313 is probably the catalytic proton donor site Bifidobacterium bifidum JCM 1254
0.06
-
 E356Q Bifidobacterium bifidum JCM 1254
0.17
-
 D29N Bifidobacterium bifidum JCM 1254
0.22
-
 D160N Bifidobacterium bifidum JCM 1254
0.31
-
 E319Q Bifidobacterium bifidum JCM 1254
0.53
-
 E568Q Bifidobacterium bifidum JCM 1254
0.72
-
 D232N Bifidobacterium bifidum JCM 1254
0.9
-
 D182N Bifidobacterium bifidum JCM 1254
1.68
-
 E259Q Bifidobacterium bifidum JCM 1254
2.85
-
 D280N Bifidobacterium bifidum JCM 1254
3.61
-
 D35N Bifidobacterium bifidum JCM 1254
3.93
-
 E377Q Bifidobacterium bifidum JCM 1254
4.79
-
 E475Q Bifidobacterium bifidum JCM 1254
5.41
-
 D491N Bifidobacterium bifidum JCM 1254
5.71
-
 D348N Bifidobacterium bifidum JCM 1254
5.73
-
 E249Q Bifidobacterium bifidum JCM 1254
5.84
-
 E691Q Bifidobacterium bifidum JCM 1254
5.9
-
 D561N Bifidobacterium bifidum JCM 1254
6.09
-
 D331N Bifidobacterium bifidum JCM 1254
6.71
-
 D490N Bifidobacterium bifidum JCM 1254
7.26
-
 D108N Bifidobacterium bifidum JCM 1254
7.32
-
 D344N Bifidobacterium bifidum JCM 1254
7.98
-
 D373N Bifidobacterium bifidum JCM 1254
9.2
-
 D260N Bifidobacterium bifidum JCM 1254
10
-
 LNBP2 Bifidobacterium bifidum JCM 1254
10.5
-
 LNBP1, all mutants display decreased activities, D313N has no activity Bifidobacterium bifidum JCM 1254

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosmine + phosphate
-
 Bifidobacterium bifidum JCM 1254 alpha-D-galactose 1-phosphate + N-acetyl-D-galactosamine
-
 ?
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine + phosphate
-
 Bifidobacterium bifidum JCM 1254 alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine
-
 ?

Synonyms

Synonyms Comment Organism
lacto-N-biose phosphorylase
-
 Bifidobacterium bifidum JCM 1254
lnba1 isoform LNBP1 Bifidobacterium bifidum JCM 1254
lnba2 isoform LNBP2 Bifidobacterium bifidum JCM 1254
LNBP
-
 Bifidobacterium bifidum JCM 1254