Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutants enzymes in Escherichia coli | Pasteurella multocida |
Protein Variants | Comment | Organism |
---|---|---|
D247E | site-directed mutagenesis, mutant possesses only GlcUA-transferase activity | Pasteurella multocida |
D247K | site-directed mutagenesis, mutant possesses only GlcUA-transferase activity | Pasteurella multocida |
D247N | site-directed mutagenesis, mutant possesses only GlcUA-transferase activity | Pasteurella multocida |
D249E | site-directed mutagenesis, mutant possesses only GlcUA-transferase activity | Pasteurella multocida |
D249K | site-directed mutagenesis, mutant possesses only GlcUA-transferase activity | Pasteurella multocida |
D249N | site-directed mutagenesis, mutant possesses only GlcUA-transferase activity | Pasteurella multocida |
D370E | site-directed mutagenesis, mutant possesses GlcUA-transferase activity, and very low hyaluronan synthase activity | Pasteurella multocida |
D370K | site-directed mutagenesis, mutant possesses GlcUA-transferase activity, and very low GlcNAc-transferase activity | Pasteurella multocida |
D370N | site-directed mutagenesis, mutant possesses GlcUA-transferase activity, and very low GlcNAc-transferase activity | Pasteurella multocida |
D527E | site-directed mutagenesis, mutant possesses only GlcNAc-transferase activity | Pasteurella multocida |
D527K | site-directed mutagenesis, mutant possesses only GlcNAc-transferase activity | Pasteurella multocida |
D527N | site-directed mutagenesis, mutant possesses only GlcNAc-transferase activity | Pasteurella multocida |
D529E | site-directed mutagenesis, mutant possesses GlcNAc-transferase activity, and low hyaluronan synthase activity | Pasteurella multocida |
D529K | site-directed mutagenesis, mutant possesses GlcNAc-transferase activity, and very low hyaluronan synthase activity | Pasteurella multocida |
D529N | site-directed mutagenesis, mutant possesses only GlcNAc-transferase activity | Pasteurella multocida |
E369D | site-directed mutagenesis, mutant possesses GlcUA-transferase activity, and very low GlcNAc-transferase activity | Pasteurella multocida |
E369H | site-directed mutagenesis, mutant possesses GlcUA-transferase activity, and very low GlcNAc-transferase activity | Pasteurella multocida |
E369Q | site-directed mutagenesis, mutant possesses GlcUA-transferase activity, and very low GlcNAc-transferase activity | Pasteurella multocida |
additional information | activity rescue of mutants with high substrate concentrations, overview, deletion of residues 1-117 does not affect polymerization activity, construction of different chimeric mutant enzymes comprising residues from Pasteurella multocida type A enzyme and residues of a Pasteurella multocida type F chondroitin synthase, producing an unsulfated chondroitin capsule, the chimeric mutants show different percentages of hyaluronan and chondroitin synthase ativities, overview | Pasteurella multocida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, wild-type and mutant enzymes | Pasteurella multocida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 18% and 39% of the wild-type GlcUA-transferase and GlcNAc-transferase activity with Mn2+, respectively, 0.2 mM | Pasteurella multocida | |
Mg2+ | 66% and 77% of the wild-type GlcUA-transferase and GlcNAc-transferase activity with Mn2+, respectively, 0.2 mM | Pasteurella multocida | |
Mn2+ | absolutely required, best metal cofactor, 0.2 mM | Pasteurella multocida | |
additional information | metal requirements of mutant enzymes, overview | Pasteurella multocida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pasteurella multocida | the produced hyaluronan capsule enhances infection | ? | - |
? | |
additional information | Pasteurella multocida type A | the produced hyaluronan capsule enhances infection | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pasteurella multocida | - |
type A | - |
Pasteurella multocida type A | - |
type A | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
UDP-N-acetyl-alpha-D-glucosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-[nascent hyaluronan] = UDP + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-[nascent hyaluronan] | 2 active sites, the 2 DXD motifs of the transferase sites in the C-terminus, residues 686-703, are essential for activity | Pasteurella multocida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the produced hyaluronan capsule enhances infection | Pasteurella multocida | ? | - |
? | |
additional information | substrate specificity, the GlcNAc-transferase, but not the GlcUA-transferase activity depends on the WGGED motif, overview | Pasteurella multocida | ? | - |
? | |
additional information | the produced hyaluronan capsule enhances infection | Pasteurella multocida type A | ? | - |
? | |
additional information | substrate specificity, the GlcNAc-transferase, but not the GlcUA-transferase activity depends on the WGGED motif, overview | Pasteurella multocida type A | ? | - |
? | |
UDP-N-acetyl-D-glucosamine + UDP-D-glucuronate | formation of linear hyaluronan polymers composed of alternating beta3-N-acetylglucosamine-beta4-glucuronic acid | Pasteurella multocida | [beta-N-acetyl-D-glucosaminyl(1-4)beta-D-glucuronosyl(1-3)]n + UDP | the product chain length can grow at the reducing end up to 40000 monosaccharides with a MW of over 8 million Da before it is released by the class I enzyme | ? | |
UDP-N-acetyl-D-glucosamine + UDP-D-glucuronate | formation of linear hyaluronan polymers composed of alternating beta3-N-acetylglucosamine-beta4-glucuronic acid | Pasteurella multocida type A | [beta-N-acetyl-D-glucosaminyl(1-4)beta-D-glucuronosyl(1-3)]n + UDP | the product chain length can grow at the reducing end up to 40000 monosaccharides with a MW of over 8 million Da before it is released by the class I enzyme | ? |
Synonyms | Comment | Organism |
---|---|---|
HA synthase | - |
Pasteurella multocida |
HAS | - |
Pasteurella multocida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pasteurella multocida |