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Literature summary for 2.4.1.212 extracted from

  • Weigel, P.H.
    Functional characteristics and catalytic mechanisms of the bacterial hyaluronan synthases (2002), IUBMB Life, 54, 201-211.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
cardiolipin required for full activity, activates up to 10fold, 14-18 molecules of cardiolipin are bound to one molecule of enzyme Streptococcus pyogenes

Cloned(Commentary)

Cloned (Comment) Organism
expression as a soluble active protein comprising residues 1-703 Pasteurella multocida
overexpression in Escherichia coli, enzyme cannot be expressed as a soluble active protein Streptococcus pyogenes

Localization

Localization Comment Organism GeneOntology No. Textmining
cell membrane most parts of the enzyme, except the transmembrane region, are cytoplasmic including the active site Streptococcus pyogenes
-
-
additional information probably cytoplasm or periplasm Pasteurella multocida
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Streptococcus pyogenes
Mg2+ required by class II enzyme Pasteurella multocida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
1 * 48000, without cardiolipin, radiation inactivation analysis Streptococcus pyogenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Streptococcus pyogenes enzyme is responsible for hyaluronan biosynthesis, the hyaluronan capsule is an important, but not the only, virulence factor, physiological role of the enzyme ?
-
?
additional information Pasteurella multocida enzyme is responsible for hyaluronan biosynthesis, the hyaluronan capsule is an important, but not the only, virulence factor, physiological role of the enzyme ?
-
?

Organism

Organism UniProt Comment Textmining
Pasteurella multocida
-
avian pathogen, class II enzyme
-
Streptococcus pyogenes
-
human pathogen, single gene hasA in the has operon, class I enzyme
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli, native enzyme to homogeneity Streptococcus pyogenes

Reaction

Reaction Comment Organism Reaction ID
UDP-N-acetyl-alpha-D-glucosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-[nascent hyaluronan] = UDP + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-[nascent hyaluronan] catalytic mechanism Pasteurella multocida
UDP-N-acetyl-alpha-D-glucosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-[nascent hyaluronan] = UDP + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-[nascent hyaluronan] catalytic mechanism, no covalent intermediates Streptococcus pyogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme is responsible for hyaluronan biosynthesis, the hyaluronan capsule is an important, but not the only, virulence factor, physiological role of the enzyme Streptococcus pyogenes ?
-
?
additional information enzyme is responsible for hyaluronan biosynthesis, the hyaluronan capsule is an important, but not the only, virulence factor, physiological role of the enzyme Pasteurella multocida ?
-
?
additional information enzyme acts processive, the enzyme is active as a complex with cardiolipin, a bacterial membrane lipid Streptococcus pyogenes ?
-
?
additional information enzyme is not processive, enzyme requires other proteins for hyaluronan translocation Pasteurella multocida ?
-
?
UDP-D-glucosamine + UDP-D-glucuronate
-
Streptococcus pyogenes [beta-D-glucosaminyl(1-4)beta-D-glucuronosyl(1-3)]n + UDP
-
?
UDP-N-acetyl-D-glucosamine + UDP-D-glucuronate addition of monosaccharides to the reducing end of the acceptor substrate, no binding and activity with exogenously added hyaluronan chains Streptococcus pyogenes [beta-N-acetyl-D-glucosaminyl(1-4)beta-D-glucuronosyl(1-3)]n + UDP the product chain length can grow at the reducing end up to 40000 monosaccharides with a MW of over 8 million Da before it is released by the class I enzyme ?
UDP-N-acetyl-D-glucosamine + UDP-D-glucuronate chains growth at the non-reducing end, which is terminated by lack of substrate with a non-reducing end, active with exogenously added acceptors substrates Pasteurella multocida [beta-N-acetyl-D-glucosaminyl(1-4)beta-D-glucuronosyl(1-3)]n + UDP
-
?

Subunits

Subunits Comment Organism
monomer 1 * 48000, without cardiolipin, radiation inactivation analysis Streptococcus pyogenes
More enzyme topology and organization, overview Streptococcus pyogenes
More predicted enzyme topology and organization, overview Pasteurella multocida

Synonyms

Synonyms Comment Organism
HA synthase
-
Streptococcus pyogenes
HA synthase
-
Pasteurella multocida
More the enzyme of Pasteurella multocida belongs to the group of class II hyaluronan synthases Pasteurella multocida
More the enzyme of Streptococcus pyogenes belongs to the group of class I hyaluronan synthases Streptococcus pyogenes