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Literature summary for 2.4.1.212 extracted from
- An enzyme capture assay for analysis of active hyaluronan synthases (2007), Anal. Biochem., 371, 62-70.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| Homo sapiens hyaluronan synthase 1 is cloned into the plasmid pFLAG-CMV2, for in vitro translation the pF3A WG(BYDV) Flexi vector is used | Homo sapiens |
| Streptococcus equisimilis hyaluronan synthase is cloned into the plasmid pKK223-3 for expression in Escherichia coli SURE cells | Streptococcus dysgalactiae subsp. equisimilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-glucuronate + N-acetyl-beta-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-[nascent hyaluronan] | Streptococcus dysgalactiae subsp. equisimilis | - |
UDP + beta-D-glucuronosyl-(1-3)-N-acetyl-beta-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-[nascent hyaluronan] | - |
? |  |
| UDP-alpha-D-glucuronate + N-acetyl-beta-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-[nascent hyaluronan] | Homo sapiens | - |
UDP + beta-D-glucuronosyl-(1-3)-N-acetyl-beta-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-[nascent hyaluronan] | - |
? | |
| UDP-alpha-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1-3)-N-acetyl-beta-D-glucosaminyl-(1-4)-[nascent hyaluronan] | Streptococcus dysgalactiae subsp. equisimilis | - |
UDP + N-acetyl-beta-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-N-acetyl-beta-D-glucosaminyl-(1-4)-[nascent hyaluronan] | - |
? | |
| UDP-alpha-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1-3)-N-acetyl-beta-D-glucosaminyl-(1-4)-[nascent hyaluronan] | Homo sapiens | - |
UDP + N-acetyl-beta-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-N-acetyl-beta-D-glucosaminyl-(1-4)-[nascent hyaluronan] | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Streptococcus dysgalactiae subsp. equisimilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| on a Ni2+-nitrilotriacetic acid resin | Streptococcus dysgalactiae subsp. equisimilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-glucuronate + N-acetyl-beta-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-[nascent hyaluronan] | - |
Streptococcus dysgalactiae subsp. equisimilis | UDP + beta-D-glucuronosyl-(1-3)-N-acetyl-beta-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-[nascent hyaluronan] | - |
? | |
| UDP-alpha-D-glucuronate + N-acetyl-beta-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-[nascent hyaluronan] | - |
Homo sapiens | UDP + beta-D-glucuronosyl-(1-3)-N-acetyl-beta-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-[nascent hyaluronan] | - |
? | |
| UDP-alpha-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1-3)-N-acetyl-beta-D-glucosaminyl-(1-4)-[nascent hyaluronan] | - |
Streptococcus dysgalactiae subsp. equisimilis | UDP + N-acetyl-beta-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-N-acetyl-beta-D-glucosaminyl-(1-4)-[nascent hyaluronan] | - |
? | |
| UDP-alpha-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1-3)-N-acetyl-beta-D-glucosaminyl-(1-4)-[nascent hyaluronan] | - |
Homo sapiens | UDP + N-acetyl-beta-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-N-acetyl-beta-D-glucosaminyl-(1-4)-[nascent hyaluronan] | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HAS | - |
Streptococcus dysgalactiae subsp. equisimilis |
| HAS | - |
Homo sapiens |
| HAS1 | - |
Homo sapiens |
| HsHAS1 | - |
Homo sapiens |
| hyaluronan synthase | - |
Streptococcus dysgalactiae subsp. equisimilis |
| hyaluronan synthase | - |
Homo sapiens |
| seHAS | - |
Streptococcus dysgalactiae subsp. equisimilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
HAS capture assay, overnight at room temperature | Streptococcus dysgalactiae subsp. equisimilis |
| 22 | - |
HAS capture assay, overnight at room temperature | Homo sapiens |
| 30 | - |
standard assay for HAS activity | Streptococcus dysgalactiae subsp. equisimilis |
| 37 | - |
standard assay for HAS activity | Homo sapiens |
| 60 | - |
HAS capture assay, 3 h | Streptococcus dysgalactiae subsp. equisimilis |
| 60 | - |
HAS capture assay, 3 h | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
HAS capture assay | Streptococcus dysgalactiae subsp. equisimilis |
| 5 | - |
HAS capture assay | Homo sapiens |
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