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Literature summary for 2.4.1.214 extracted from

  • Staudacher, E.; Altmann, F.; Glossl, J.; M"rz, L.; Schachter, H.; Kamerling, J.P.; Hård, K.; Vliegenthart, J.F.G.
    GDP-fucose:beta-N-acetylglucosamine (Fuc to (Fucalpha1-6GlcNAc)-Asn-peptide) alpha1-3-fucosyltransferase activity in honeybee (Apis mellifica) venom glands. The difucosylation of asparagine-bound N-acetylglucosamine (1991), Eur. J. Biochem., 199, 745-751.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Mn2+ at concentrations above 40 mM Apis mellifera
Triton X-100 at concentrations above 0.5% Apis mellifera

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.7
-
GlcNAcbeta(1->2)Manalpha(1->6)(GlcNAcbeta(1->2)Manalpha(1->3))Manbeta(1->4)GlcNAcbeta(1->4)(ucalpha(1->6)G)cNAcbeta(1->)N-Asn-peptide(NAc) GnGn + F(Ac) Apis mellifera

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ required, optimal activity at 20 mM Apis mellifera

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine Apis mellifera
-
GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-3)]-N-acetyl-beta-D-glucosaminyl]asparagine
-
?

Organism

Organism UniProt Comment Textmining
Apis mellifera
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
venom gland
-
Apis mellifera
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-beta-L-fucose + GlcNAc-beta-(1->2)-Man-alpha-(1->6)-[GlcNAc-beta-(1->2)-Man-alpha-(1->3)]-Man-beta-(1->4)-GlcNAc-beta-(1->4)-[(Fuc-alpha-(1->6)]-GlcNAc-beta-(1->)-N?Asn?peptide
-
Apis mellifera GDP + GlcNAc-beta-(1->2)-Man-alpha-(1->6)-[GlcNAc-beta-(1->2)-Man-alpha-(1->3)]-Man-beta-(1->4)-GlcNAc-beta-(1->4)-[(Fuc-alpha-(1->6)]-[(Fuc-alpha-(1->3)]-GlcNAc-beta-(1->)-N?Asn?peptide
-
?
GDP-beta-L-fucose + GlcNAcbeta(1->2)Manalpha(1->6)(GlcNAcbeta(1->2)Manalpha(1->3))Manbeta(1->4)GlcNAcbeta(1->4)(ucalpha(1->6)G)cNAcbeta(1->)N-Asn-peptide(NAc)
-
Apis mellifera ?
-
?
GDP-fucose + GlcNAcbeta1-2-Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4(Fucalpha1-6)GlcNAcbeta1-N-Asn-peptide(Nac)
-
Apis mellifera GDP + GlcNAcbeta1-2-Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4(Fucalpha1-6)(Fucalpha1-3)GlcNAcbeta1-N-Asn-peptide(Nac) the product shows a difucosylated structure with two Fuc residues at the Asn-bound GlcNAc residue, 40% yield ?
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine
-
Apis mellifera GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-3)]-N-acetyl-beta-D-glucosaminyl]asparagine
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
Apis mellifera