Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.214 extracted from

  • Jost, F.; de Vries, T.; Knegtel, R.M.; Macher, B.A.
    Mutation of amino acids in the alpha 1,3-fucosyltransferase motif affects enzyme activity and Km for donor and acceptor substrates (2005), Glycobiology, 15, 165-175.
    View publication on PubMed
Search for more literature for 2.4.1.214

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant FucT VIs in COS-7 cells as soluble N-terminal protein A-fusion proteins, mutant K241A shows a low expression level Homo sapiens

Protein Variants

Protein Variants Comment Organism
E247A site-directed mutagenesis of a residue from the conserved alpha 1,3 FucT motif, below 1% activity compared to the wild-type FucT VI Homo sapiens
E257A site-directed mutagenesis of a residue from the conserved alpha 1,3 FucT motif, below 1% activity compared to the wild-type FucT VI Homo sapiens
F242A site-directed mutagenesis of a residue from the conserved alpha 1,3 FucT motif, below 1% activity compared to the wild-type FucT VI Homo sapiens
F246A site-directed mutagenesis of a residue from the conserved alpha 1,3 FucT motif, 7% activity compared to the wild-type FucT VI Homo sapiens
K241A site-directed mutagenesis of a residue from the conserved alpha 1,3 FucT motif, low expression level in COS-7 cells, below 1% activity compared to the wild-type FucT VI Homo sapiens
K258A site-directed mutagenesis of a residue from the conserved alpha 1,3 FucT motif, inactive mutant Homo sapiens
L244A site-directed mutagenesis of a residue from the conserved alpha 1,3 FucT motif, 7% activity compared to the wild-type FucT VI Homo sapiens
N248A site-directed mutagenesis of a residue from the conserved alpha 1,3 FucT motif, below 1% activity compared to the wild-type FucT VI Homo sapiens
S249A site-directed mutagenesis of a residue from the conserved alpha 1,3 FucT motif, 57% activity compared to the wild-type FucT VI Homo sapiens
T256A site-directed mutagenesis of a residue from the conserved alpha 1,3 FucT motif, below 1% activity compared to the wild-type FucT VI Homo sapiens
Y240A site-directed mutagenesis of a residue from the conserved alpha 1,3 FucT motif, 14% activity compared to the wild-type FucT VI Homo sapiens
Y254A site-directed mutagenesis of a residue from the conserved alpha 1,3 FucT motif, inactive mutant Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics of wild-type and alpha 1,3 FucT motif mutants Homo sapiens
0.019
-
 GDP-L-fucose pH 6.5, recombinant wild-type FucT VI Homo sapiens
0.069
-
 GDP-L-fucose pH 6.5, recombinant FucT VI mutant S249A Homo sapiens
0.11
-
 GDP-L-fucose pH 6.5, recombinant FucT VI mutant F246A Homo sapiens
0.13
-
 LacNAc-C8 pH 6.5, recombinant FucT VI mutants Y240A and L244A Homo sapiens
0.15
-
 LacNAc-C8 pH 6.5, recombinant FucT VI mutant F246A Homo sapiens
0.22
-
 GDP-L-fucose pH 6.5, recombinant FucT VI mutant Y240A Homo sapiens
0.22
-
 LacNAc-C8 pH 6.5, recombinant wild-type FucT VI Homo sapiens
0.336
-
 GDP-L-fucose pH 6.5, recombinant FucT VI mutant L244A Homo sapiens
1.6
-
 LacNAc-C8 pH 6.5, recombinant FucT VI mutant S249A Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+
-
 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant soluble protein A-fusion wild-type and mutant FucT VIs from COS-7 cells by IgG affinity chromatography Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
GDP-beta-L-fucose + N4-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc]-L-asparaginyl-[protein] = GDP + N4-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-[alpha-L-Fuc-(1->3)]-beta-D-GlcNAc]-L-asparaginyl-[protein] the enzyme contains the conserved alpha 1,3 FucT motif, which is crucial for activity and nucleotide sugar binding Homo sapiens
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-L-fucose + LacNAc-C8
-
 Homo sapiens GDP + alpha1,3-fucosylated LacNAc-C8
-
 ?
additional information substrate specificities of wild-type and alpha 1,3 FucT motif mutants, overview Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
alpha 1,3-fucosyltransferase VI
-
 Homo sapiens
FucT VI
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 assay at Homo sapiens