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Literature summary for 2.4.1.214 extracted from

  • Sun, H.Y.; Lin, S.W.; Ko, T.P.; Pan, J.F.; Liu, C.L.; Lin, C.N.; Wang, A.H.; Lin, C.H.
    Structure and mechanism of Helicobacter pylori fucosyltransferase. A basis for lipopolysaccharide variation and inhibitor design (2007), J. Biol. Chem., 282, 9973-9982.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression Escherichia coli to obtain a selenomethionine-labeled L202M FucT for crystallization Helicobacter pylori

Crystallization (Commentary)

Crystallization (Comment) Organism
by the hanging drop vapor diffusion method, as FucT, FucT-GDPfucose and FucT-GDP complexes Helicobacter pylori

Protein Variants

Protein Variants Comment Organism
E249A FucT 45, no activity Helicobacter pylori
E249D FucT 45, no activity Helicobacter pylori
E249Q FucT 45, no activity Helicobacter pylori
E95A FucT 45, no activity Helicobacter pylori
E95D FucT 45, no activity Helicobacter pylori
K250A FucT 45, no activity Helicobacter pylori
L202M to introduce methionine Helicobacter pylori
N240D FucT 45, active Helicobacter pylori
R195A FucT 45, no activity Helicobacter pylori
R195K FucT 45, active Helicobacter pylori
Y246A FucT 45, active Helicobacter pylori
Y246F FucT 45, active Helicobacter pylori

Inhibitors

Inhibitors Comment Organism Structure
GDP-hexylamine
-
Helicobacter pylori
GDP-N-(biphenyl-4-ylmethyl)-5-(1H-1,2,3-triazol-1-yl)pentanamide
-
Helicobacter pylori
GDP-propylamine
-
Helicobacter pylori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08016
-
GDP-fucose FucT 45, N240A mutant Helicobacter pylori
0.08831
-
GDP-fucose FucT 45 Helicobacter pylori
0.157
-
GDP-fucose FucT 45, Y246F mutant Helicobacter pylori
0.2223
-
GDP-fucose FucT 115 Helicobacter pylori
0.59 1 GDP-fucose FucT 45, R195K mutant Helicobacter pylori
0.71
-
N-acetyllactosamine FucT 45 Helicobacter pylori
0.8
-
GDP-fucose FucT 45, Y246A mutant Helicobacter pylori
1.29
-
N-acetyllactosamine FucT 45, Y246F mutant Helicobacter pylori
2.39
-
N-acetyllactosamine FucT 45, Y246A mutant Helicobacter pylori
8.92
-
N-acetyllactosamine FucT 115 Helicobacter pylori
10.35
-
N-acetyllactosamine FucT 45, R195K mutant Helicobacter pylori
12.77
-
N-acetyllactosamine FucT 45, N240A mutant Helicobacter pylori

Organism

Organism UniProt Comment Textmining
Helicobacter pylori
-
-
-
Helicobacter pylori NCTC11639
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-fucose + N-acetyllactosamine FucT involved in production of the Lewis x trisaccharide, the major component of the bacteria’s lipopolysaccharides Helicobacter pylori GDP + Galbeta1-4(Fucalpha1-3)GlcNAc
-
?
GDP-fucose + N-acetyllactosamine FucT involved in production of the Lewis x trisaccharide, the major component of the bacteria’s lipopolysaccharides Helicobacter pylori NCTC11639 GDP + Galbeta1-4(Fucalpha1-3)GlcNAc
-
?

Synonyms

Synonyms Comment Organism
alpha1,3-fucosyltransferase
-
Helicobacter pylori
FucT
-
Helicobacter pylori
FucT 115 truncated FucT with C-terminal 115 residues Helicobacter pylori
FucT 45 truncated FucT with C-terminal 45 residues Helicobacter pylori

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00059
-
GDP-N-(biphenyl-4-ylmethyl)-5-(1H-1,2,3-triazol-1-yl)pentanamide
-
Helicobacter pylori