Cloned (Comment) | Organism |
---|---|
overexpression Escherichia coli to obtain a selenomethionine-labeled L202M FucT for crystallization | Helicobacter pylori |
Crystallization (Comment) | Organism |
---|---|
by the hanging drop vapor diffusion method, as FucT, FucT-GDPfucose and FucT-GDP complexes | Helicobacter pylori |
Protein Variants | Comment | Organism |
---|---|---|
E249A | FucT 45, no activity | Helicobacter pylori |
E249D | FucT 45, no activity | Helicobacter pylori |
E249Q | FucT 45, no activity | Helicobacter pylori |
E95A | FucT 45, no activity | Helicobacter pylori |
E95D | FucT 45, no activity | Helicobacter pylori |
K250A | FucT 45, no activity | Helicobacter pylori |
L202M | to introduce methionine | Helicobacter pylori |
N240D | FucT 45, active | Helicobacter pylori |
R195A | FucT 45, no activity | Helicobacter pylori |
R195K | FucT 45, active | Helicobacter pylori |
Y246A | FucT 45, active | Helicobacter pylori |
Y246F | FucT 45, active | Helicobacter pylori |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GDP-hexylamine | - |
Helicobacter pylori | |
GDP-N-(biphenyl-4-ylmethyl)-5-(1H-1,2,3-triazol-1-yl)pentanamide | - |
Helicobacter pylori | |
GDP-propylamine | - |
Helicobacter pylori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08016 | - |
GDP-fucose | FucT 45, N240A mutant | Helicobacter pylori | |
0.08831 | - |
GDP-fucose | FucT 45 | Helicobacter pylori | |
0.157 | - |
GDP-fucose | FucT 45, Y246F mutant | Helicobacter pylori | |
0.2223 | - |
GDP-fucose | FucT 115 | Helicobacter pylori | |
0.59 | 1 | GDP-fucose | FucT 45, R195K mutant | Helicobacter pylori | |
0.71 | - |
N-acetyllactosamine | FucT 45 | Helicobacter pylori | |
0.8 | - |
GDP-fucose | FucT 45, Y246A mutant | Helicobacter pylori | |
1.29 | - |
N-acetyllactosamine | FucT 45, Y246F mutant | Helicobacter pylori | |
2.39 | - |
N-acetyllactosamine | FucT 45, Y246A mutant | Helicobacter pylori | |
8.92 | - |
N-acetyllactosamine | FucT 115 | Helicobacter pylori | |
10.35 | - |
N-acetyllactosamine | FucT 45, R195K mutant | Helicobacter pylori | |
12.77 | - |
N-acetyllactosamine | FucT 45, N240A mutant | Helicobacter pylori |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicobacter pylori | - |
- |
- |
Helicobacter pylori NCTC11639 | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-fucose + N-acetyllactosamine | FucT involved in production of the Lewis x trisaccharide, the major component of the bacterias lipopolysaccharides | Helicobacter pylori | GDP + Galbeta1-4(Fucalpha1-3)GlcNAc | - |
? | |
GDP-fucose + N-acetyllactosamine | FucT involved in production of the Lewis x trisaccharide, the major component of the bacterias lipopolysaccharides | Helicobacter pylori NCTC11639 | GDP + Galbeta1-4(Fucalpha1-3)GlcNAc | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha1,3-fucosyltransferase | - |
Helicobacter pylori |
FucT | - |
Helicobacter pylori |
FucT 115 | truncated FucT with C-terminal 115 residues | Helicobacter pylori |
FucT 45 | truncated FucT with C-terminal 45 residues | Helicobacter pylori |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00059 | - |
GDP-N-(biphenyl-4-ylmethyl)-5-(1H-1,2,3-triazol-1-yl)pentanamide | - |
Helicobacter pylori |