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Literature summary for 2.4.1.221 extracted from
- Purification and characterization of a GDP-fucose:polypeptide fucosyltransferase from Chinese hamster ovary cells (1998), J. Biol. Chem., 273, 8112-8118.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| factor VII EGF-1 | inhibition above 0.015 mM | Cricetulus griseus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.006 | - |
GDP-beta-L-fucose | - |
Cricetulus griseus |  |
| 0.015 | - |
factor VII EGF | - |
Cricetulus griseus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | 17fold increase at 50 mM | Cricetulus griseus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44000 | - |
SDS-PAGE | Cricetulus griseus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cricetulus griseus | - |
Chinese hamster, CHO cells | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | glycoprotein with more than one high mannose type oligosaccharide chain | Cricetulus griseus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cricetulus griseus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| CHO cell | - |
Cricetulus griseus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.784 | - |
- |
Cricetulus griseus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP-beta-L-fucose + factor VII EGF | - |
Cricetulus griseus | ? | - |
? | |
| additional information | fucosylates various synthetic peptides of EGF-1 domain of human factor VII, GDP-mannose, UDP-glucose, UDP-N-acetylglucosamine, UDP-galactose, UDP-H-acetylgalactosamine and UDP-xylose can replace GDP-fucose | Cricetulus griseus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Cricetulus griseus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Cricetulus griseus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8 | - |
Cricetulus griseus |
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