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Literature summary for 2.4.1.226 extracted from

  • Yada, T.; Gotoh, M.; Sato, T.; Shionyu, M.; Go, M.; Kaseyama, H.; Iwasaki, H.; Kikuchi, N.; Kwon, Y.D.; Togayachi, A.; Kudo, T.; Watanabe, H.; Narimatsu, H.; Kimata, K.
    Chondroitin sulfate synthase-2. Molecular cloning and characterization of a novel human glycosyltransferase homologous to chondroitin sulfate glucuronyltransferase, which has dual enzymatic activities (2003), J. Biol. Chem., 278, 30235-30247.
    View publication on PubMed
Search for more literature for 2.4.1.226

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression of putative catalytic domains of CSGlcAT-II and CSS2 in COS-7 cells as FLAG-tagged soluble proteins, which possesses glucuronyltransferase and N-acetylgalactosaminyltransferase activities as the wild-type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.027
-
 C11-oligosaccharide of chondroitin sulfate A pH 6.2, 37°C, CSS2 Homo sapiens
0.263
-
 UDP-alpha-D-glucuronate pH 6.2, 37°C, CSS2 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane enzyme is a type II membrane protein Homo sapiens 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 17% and 31% as effective as Mn2+ for glucuronyltransferase and N-acetylgalactosaminyltransferase activities, respectively Homo sapiens
Mn2+ best metal ion, optimal concentration is 10 mM for both activities Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q8IZ52 chondroitin sulfate synthase-2, i.e. CSS2; 2 enzymes: CSGlcAT-II and CSS2
-

Purification (Commentary)

Purification (Comment) Organism
recombinant FLAG-tagged soluble catalytic domain from COS-7 cells Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
UDP-alpha-D-glucuronate + [protein]-3-O-(beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine = UDP + [protein]-3-O-(beta-D-GlcA-(1->3)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine enzyme has dual function possessing both glucuronyltransferase and N-acetylgalactosaminyltransferase activities Homo sapiens
a

Source Tissue

Source Tissue Comment Organism Textmining
additional information CSS2 is ubiquitously expressed Homo sapiens
-
ovary CSS2, high level expression Homo sapiens
-
pancreas CSS2, high level expression Homo sapiens
-
placenta CSS2, high level expression Homo sapiens
-
small intestine CSS2, high level expression Homo sapiens
-
stomach CSS2, high level expression Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate specificities of enzymes CSGlcAT-II and CSS2 in truncated form Homo sapiens ?
-
 ?
UDP-alpha-D-glucuronate + C11-oligosaccharide of chondroitin sulfate A
-
 Homo sapiens ?
-
 ?
UDP-alpha-D-glucuronate + chondroitin and oligosaccharide of chondroitin with chain length of C11 Homo sapiens UDP + beta-D-glucuronosyl-(1,3)-chondroitin
-
 ?
UDP-alpha-D-glucuronate + chondroitin sulfate A and oligosaccharide of chondroitin sulfate with chain length of C11 Homo sapiens UDP + beta-D-glucuronosyl-(1,3)-chondroitin sulfate A
-
 ?

Subunits

Subunits Comment Organism
More structure modeling of the C-terminus Homo sapiens

Synonyms

Synonyms Comment Organism
chondroitin sulfate glucuronyltransferase
-
 Homo sapiens
chondroitin sulfate synthase-2
-
 Homo sapiens
CSGlcAT-II
-
 Homo sapiens
CSS2
-
 Homo sapiens
GlcAT-II
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
 assay at Homo sapiens