Cloned (Comment) | Organism |
---|---|
gene chsy1, real-time PCR enzyme expression analysis | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | gene silencing via shRNA sequences targeting Rattus norvegicus Smad2, Smad3, CHSY1, c-jun and Sp1, the shRNAs are transfected using a lentiviral vector | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucuronate + N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan | Rattus norvegicus | - |
UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
gene CHSY1 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
nucleus pulposus | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucuronate + N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan | - |
Rattus norvegicus | UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan | - |
? |
Synonyms | Comment | Organism |
---|---|---|
chondroitin sulfate synthase 1 | - |
Rattus norvegicus |
CHSY1 | - |
Rattus norvegicus |
Organism | Comment | Expression |
---|---|---|
Rattus norvegicus | knockdown of c-Jun and Sp1 decreases CHSY1 promoter activity, CHSY1 expression and sulfated glycosaminoglycan accumulation induced by TGF-beta | down |
Rattus norvegicus | TGF-beta induces up-regulation of CHSY1 in nucleus pulposus cells through MAPK signaling. TGF-beta increases CHSY1 expression and promoter activity in the nucleus pulposus partially through activation of canonical Smad signaling pathway | up |
General Information | Comment | Organism |
---|---|---|
malfunction | silencing of CHSY1 decreases sulfated glycosaminoglycan accumulation in nucleus pulposus cells induced by TGF-beta | Rattus norvegicus |
physiological function | chondroitin sulfate synthase 1 (CHSY1) is a glycosyltransferases involved in the biosynthesis of chondroitin and dermatan sulfate glycosaminoglycan. CHSY1 possesses dual glucuronyl-transferase and galactosaminyl-transferase activity. It can promote the transfer of both GlcUA and GalNAc from their donors UDP-GlcUA and UDP-GalNAc, respectively, to the non-reducing end of the chondroitin polymer, resulting in the elongating and accumulation of glycosaminoglycans | Rattus norvegicus |