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Literature summary for 2.4.1.227 extracted from

  • Hu, Y.; Chen, L.; Ha, S.; Gross, B.; Falcone, B.; Walker, D.; Mokhtarzadeh, M.; Walker, S.
    Crystal structure of the MurG: UDP-GlcNAc complex reveals common structural principles of a superfamily of glycosyltransferases (2003), Proc. Natl. Acad. Sci. USA, 100, 845-849.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.227

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli cells BL21-DE3-pLysS Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapor-diffusion method, X-ray structure Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.053
-
 UDP-GlcNAc
-
 Escherichia coli
0.053
-
 synthetic substrate analogue 7
-
 Escherichia coli
1.5
-
 2'-dUDP-N-acetyl-alpha-D-glucosamine
-
 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P17443
-
-

Storage Stability

Storage Stability Organism
-70°C Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dUDP-N-acetyl-alpha-D-glucosamine + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
 Escherichia coli ?
-
 ?
additional information no substrates: TDP-GlcNAc, C4 hydroxyl of UDP-GalNAc cannot form hydrogen bonds to A264 or N292 Escherichia coli ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
14
-
 synthetic substrate analogue 7
-
 Escherichia coli