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Literature summary for 2.4.1.227 extracted from

  • van den Brink-van der Laan, E.; Boots, J.W.P.; Spelbrink, R.E.J.; Kool, G.M.; Breukink, E.; Killian, J.A.; de Kruijff, B.
    Membrane interaction of the glycosyltransferase MurG: A special role for cardiolipin (2003), J. Bacteriol., 185, 3773-3779.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.227

Application

Application Comment Organism
synthesis overexpression of enzyme results in formation of vesicular intracellular membranes enriched in cardiolipin. Cardiolipin content of cell is about 7fold increased Escherichia coli

General Stability

General Stability Organism
additon of 20% glycerol required for storage at -20°C Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 x * 38000, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli role of enzyme in peptidoglycan biosynthesis pathway ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
when overexpressed, enzyme copurifies with lipid vesicles deriving from intracellular vesicular mebranes Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 enzyme activity is higher in vesicles containing cardiolipin than for vesicles with phosphatidylglycerol Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information role of enzyme in peptidoglycan biosynthesis pathway Escherichia coli ?
-
 ?
UDP-N-acetylglucosamine + lipid I i.e. Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol Escherichia coli UDP + lipid II i.e. N-acetylglucosamine-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol ?

Subunits

Subunits Comment Organism
? x * 38000, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
MurG
-
 Escherichia coli