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Literature summary for 2.4.1.227 extracted from
- Role of the amino acid invariants in the active site of MurG as evaluated by site-directed mutagenesis (2007), Biochimie, 89, 1498-1508.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into the vector pQE60 for expression of MurG in Escherichia coli JM109 cells | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E125A | amino acid substitution for evaluation of its role in the active site of MurG | Escherichia coli |
| E268A | amino acid substitution for evaluation of its role in the active site of MurG | Escherichia coli |
| H124A | amino acid substitution for evaluation of its role in the active site of MurG | Escherichia coli |
| H18A | amino acid substitution for evaluation of its role in the active site of MurG | Escherichia coli |
| N127A | amino acid substitution for evaluation of its role in the active site of MurG | Escherichia coli |
| N134A | amino acid substitution for evaluation of its role in the active site of MurG | Escherichia coli |
| N198A | amino acid substitution for evaluation of its role in the active site of MurG | Escherichia coli |
| N291A | amino acid substitution for evaluation of its role in the active site of MurG | Escherichia coli |
| Q288A | amino acid substitution for evaluation of its role in the active site of MurG | Escherichia coli |
| R260A | amino acid substitution for evaluation of its role in the active site of MurG | Escherichia coli |
| S191A | amino acid substitution for evaluation of its role in the active site of MurG | Escherichia coli |
| T15A | amino acid substitution for evaluation of its role in the active site of MurG | Escherichia coli |
| Y105A | amino acid substitution for evaluation of its role in the active site of MurG | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0028 | - |
MurNAc(Neta-dansylpentapeptide)-diphosphoryl (R,S-alpha-dihydroheptaprenol) | wild-type enzyme | Escherichia coli |  |
| 0.0029 | - |
MurNAc(Neta-dansylpentapeptide)-diphosphoryl (R,S-alpha-dihydroheptaprenol) | mutant R260A | Escherichia coli | |
| 0.0042 | - |
MurNAc(Neta-dansylpentapeptide)-diphosphoryl (R,S-alpha-dihydroheptaprenol) | mutant N198A | Escherichia coli | |
| 0.0055 | - |
MurNAc(Neta-dansylpentapeptide)-diphosphoryl (R,S-alpha-dihydroheptaprenol) | mutant T15A | Escherichia coli | |
| 0.0059 | - |
MurNAc(Neta-dansylpentapeptide)-diphosphoryl (R,S-alpha-dihydroheptaprenol) | mutant Y105A | Escherichia coli | |
| 0.0071 | - |
MurNAc(Neta-dansylpentapeptide)-diphosphoryl (R,S-alpha-dihydroheptaprenol) | mutant S191A | Escherichia coli | |
| 0.0085 | - |
MurNAc(Neta-dansylpentapeptide)-diphosphoryl (R,S-alpha-dihydroheptaprenol) | mutant N291A | Escherichia coli | |
| 0.009 | - |
MurNAc(Neta-dansylpentapeptide)-diphosphoryl (R,S-alpha-dihydroheptaprenol) | mutant H124A | Escherichia coli | |
| 0.0098 | - |
UDP-GlcNAc | mutant N291A | Escherichia coli | |
| 0.011 | - |
MurNAc(Neta-dansylpentapeptide)-diphosphoryl (R,S-alpha-dihydroheptaprenol) | mutant E125A | Escherichia coli | |
| 0.012 | - |
UDP-GlcNAc | mutant E125A | Escherichia coli | |
| 0.027 | - |
UDP-GlcNAc | wild-type enzyme | Escherichia coli | |
| 0.033 | - |
UDP-GlcNAc | mutant R260A | Escherichia coli | |
| 0.038 | - |
UDP-GlcNAc | mutant T15A | Escherichia coli | |
| 0.047 | - |
UDP-GlcNAc | mutant H124A | Escherichia coli | |
| 0.05 | - |
UDP-GlcNAc | mutant Y105A | Escherichia coli | |
| 0.056 | - |
UDP-GlcNAc | mutant N198A | Escherichia coli | |
| 1.03 | - |
UDP-GlcNAc | mutant S191A | Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasmic membrane | - |
Escherichia coli | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
determined by SDS-PAGE | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetylglucosamine + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol | Escherichia coli | - |
UDP + GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P17443 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using Ni2+-nitrilotriacetate-agarose | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.000019 | - |
mutant N127A | Escherichia coli |
| 0.000063 | - |
mutant E268A | Escherichia coli |
| 0.00026 | - |
mutant H18A | Escherichia coli |
| 0.00028 | - |
mutant Q288A | Escherichia coli |
| 0.00043 | - |
mutant E125A | Escherichia coli |
| 0.00053 | - |
mutant T15A | Escherichia coli |
| 0.00067 | - |
mutant H124A | Escherichia coli |
| 0.0023 | - |
mutant R260A | Escherichia coli |
| 0.0025 | - |
mutant S191A | Escherichia coli |
| 0.0064 | - |
mutant N291A | Escherichia coli |
| 0.0135 | - |
mutant N198A | Escherichia coli |
| 0.071 | - |
mutant Y105A | Escherichia coli |
| 0.37 | - |
wild type enzyme | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetylglucosamine + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol | - |
Escherichia coli | UDP + GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol | - |
? | |
| UDP-N-acetylglucosamine + MurNAc(Neta-dansylpentapeptide)-diphosphoryl (R,S-alpha-dihydroheptaprenol) | - |
Escherichia coli | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MurG | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
MurG activity assay | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.001 | - |
UDP-GlcNAc | mutant T15A | Escherichia coli | |
| 0.0016 | - |
UDP-GlcNAc | mutant E125A | Escherichia coli | |
| 0.0016 | - |
UDP-GlcNAc | mutant H124A | Escherichia coli | |
| 0.0045 | - |
UDP-GlcNAc | mutant R260A | Escherichia coli | |
| 0.0055 | - |
UDP-GlcNAc | mutant N291A | Escherichia coli | |
| 0.038 | - |
UDP-GlcNAc | mutant N198A | Escherichia coli | |
| 0.093 | - |
UDP-GlcNAc | mutant S191A | Escherichia coli | |
| 0.123 | - |
UDP-GlcNAc | mutant Y105A | Escherichia coli | |
| 0.72 | - |
UDP-GlcNAc | wild type enzyme | Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
MurG activity assay | Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9.5 | - |
Escherichia coli |
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