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Literature summary for 2.4.1.230 extracted from
- Enzymatic properties of recombinant kojibiose phosphorylase from Caldicellulosiruptor saccharolyticus ATCC43494 (2011), Biosci. Biotechnol. Biochem., 75, 1208-1210.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, expression in Escherichia coli | Caldicellulosiruptor saccharolyticus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 81000 | - |
2 * 81000, recombinant enzyme, SDS-PAGE | Caldicellulosiruptor saccharolyticus |
| 170000 | - |
recombinant enzyme, gel filtration | Caldicellulosiruptor saccharolyticus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-alpha-D-glucosyl-D-glucose + phosphate | Caldicellulosiruptor saccharolyticus | - |
D-glucose + beta-D-glucose 1-phosphate | - |
r |  |
| 2-alpha-D-glucosyl-D-glucose + phosphate | Caldicellulosiruptor saccharolyticus ATCC 43494 | - |
D-glucose + beta-D-glucose 1-phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Caldicellulosiruptor saccharolyticus | - |
- |
- |
| Caldicellulosiruptor saccharolyticus ATCC 43494 | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 31.1 | - |
purified recombinant enzyme, pH 6.0, 50°C, phosphorolysis | Caldicellulosiruptor saccharolyticus |
| 191.3 | - |
purified recombinant enzyme, pH 6.0, 50°C, synthesis | Caldicellulosiruptor saccharolyticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-alpha-D-glucosyl-D-glucose + phosphate | - |
Caldicellulosiruptor saccharolyticus | D-glucose + beta-D-glucose 1-phosphate | - |
r | |
| 2-alpha-D-glucosyl-D-glucose + phosphate | - |
Caldicellulosiruptor saccharolyticus ATCC 43494 | D-glucose + beta-D-glucose 1-phosphate | - |
r | |
| kojitetraose + phosphate | - |
Caldicellulosiruptor saccharolyticus | beta-D-glucose 1-phosphate + kojitriose | - |
r | |
| kojitetraose + phosphate | - |
Caldicellulosiruptor saccharolyticus ATCC 43494 | beta-D-glucose 1-phosphate + kojitriose | - |
r | |
| kojitriose + phosphate | - |
Caldicellulosiruptor saccharolyticus | beta-D-glucose 1-phosphate + 2-alpha-D-glucosyl-D-glucose | - |
r | |
| kojitriose + phosphate | - |
Caldicellulosiruptor saccharolyticus ATCC 43494 | beta-D-glucose 1-phosphate + 2-alpha-D-glucosyl-D-glucose | - |
r | |
| additional information | the enzyme is absolutely specific for kojioligosaccharides in the phosphorolysis reaction, no activity with lactose, sucrose, trehalose, cellobiose, isomaltose, maltose, nigerose, and neotrehalose. For the synthesis reaction, the enzyme is specific for D-glucose, followed by kojibiose and kojitriose, overview | Caldicellulosiruptor saccharolyticus | ? | - |
? | |
| additional information | the enzyme is absolutely specific for kojioligosaccharides in the phosphorolysis reaction, no activity with lactose, sucrose, trehalose, cellobiose, isomaltose, maltose, nigerose, and neotrehalose. For the synthesis reaction, the enzyme is specific for D-glucose, followed by kojibiose and kojitriose, overview | Caldicellulosiruptor saccharolyticus ATCC 43494 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 81000, recombinant enzyme, SDS-PAGE | Caldicellulosiruptor saccharolyticus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
synthesis | Caldicellulosiruptor saccharolyticus |
| 85 | - |
phosphorolysis | Caldicellulosiruptor saccharolyticus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
recombinant enzyme, stable up to, synthesis | Caldicellulosiruptor saccharolyticus |
| 85 | - |
recombinant enzyme, stable up to, phosphorolysis | Caldicellulosiruptor saccharolyticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
synthesis | Caldicellulosiruptor saccharolyticus |
| 6 | - |
phosphorolysis | Caldicellulosiruptor saccharolyticus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 10 | recombinant enzyme, stable at, phosphorolysis | Caldicellulosiruptor saccharolyticus |
| 5.5 | 10.5 | recombinant enzyme, stable at, synthesis | Caldicellulosiruptor saccharolyticus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the glycoside hydrolase family 65, GH65 | Caldicellulosiruptor saccharolyticus |
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