Any feedback?
Literature summary for 2.4.1.237 extracted from
- UDP-glucose:flavonol 7-O-glucosyltransferase activity in flower extracts of Chrysanthemum segetum (1997), Z. Naturforsch. C, 52, 153-158.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| bovine serum albumin | addition to the assay system causes 2.5fold higher reaction rate | Glebionis segetum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 1 mM, 98% inhibition with quercetin as substrate, 85% inhibition with gossypetin as substrate | Glebionis segetum |  |
| Cu2+ | 1 mM, 99% inhibition with quercetin as substrate | Glebionis segetum | |
| diethyldicarbonate | 5 mM, 98% inhibition with quercetin or gossypetin as substrate | Glebionis segetum | |
| Fe2+ | 1 mM, 98% inhibition with quercetin as substrate | Glebionis segetum | |
| Hg2+ | 1 mM, 99% inhibition with quercetin as substrate | Glebionis segetum | |
| additional information | not inhibited by EDTA | Glebionis segetum | |
| N-ethylmaleimide | strong, 1 mM, 98% inhibition with quercetin as substrate, 14 mM 2-mercaptoethanol protects, but addition after 15 min does not restore activity | Glebionis segetum | |
| p-hydroxymercuribenzoate | strong, 0.1 mM, 99.3% inhibition with quercetin as substrate, 14 mM 2-mercaptoethanol protects and addition after 15 min also reduces the inhibitory effect | Glebionis segetum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.029 | - |
kaempferol | pH 7.8, 25°C | Glebionis segetum | |
| 0.032 | - |
gossypetin | pH 6.9, 25°C | Glebionis segetum | |
| 0.049 | - |
quercetin | pH 8.3, 25°C | Glebionis segetum | |
| 0.067 | - |
myricetin | pH 8.1, 25°C | Glebionis segetum | |
| 0.071 | - |
eriodictyol | pH 7.5, 25°C | Glebionis segetum | |
| 0.073 | - |
naringenin | pH 6.9, 25°C | Glebionis segetum | |
| 0.078 | - |
apigenin | pH 7.7, 25°C | Glebionis segetum | |
| 0.084 | - |
luteolin | pH 8.1, 25°C | Glebionis segetum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | slight stimulation | Glebionis segetum | |
| KCN | slight stimulation | Glebionis segetum | |
| Mg2+ | slight stimulation | Glebionis segetum | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-glucose + quercetin | Glebionis segetum | gossypetin and quercetin are presumably the natural substrates | UDP + quercetin 7-O-beta-D-glucoside | - |
? | |
| UDP-glucose + gossypetin | Glebionis segetum | gossypetin and quercetin are presumably the natural substrates | UDP + gossypetin 7-O-beta-D-glucoside | gossypetin 7-O-glucoside is particularly responsible for the yellow coloration of the petals | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Glebionis segetum | - |
cv. Prado and Stern des Orients | - |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| UDP-glucose + a flavonol = UDP + a flavonol 7-O-beta-D-glucoside | acts on the flavonols gossypetin (8-hydroxyquercetin) and to a lesser extend on quercetin, kaempferol and myricetin | Glebionis segetum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| petal | - |
Glebionis segetum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
specific activities for the 7-O-glucosylation reaction with several substrates, cv. Stern des Orients: 3times higher specific activity with gossypetin than with quercetin as substrate | Glebionis segetum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-glucose + kaempferol | cv. Prado, gossypetin is the best substrate, followed by quercetin, kaempferol and myricetin | Glebionis segetum | UDP + kaempferol 7-O-beta-D-glucoside | - |
? | |
| UDP-alpha-D-glucose + quercetin | gossypetin and quercetin are presumably the natural substrates | Glebionis segetum | UDP + quercetin 7-O-beta-D-glucoside | - |
? | |
| UDP-alpha-D-glucose + quercetin | cv. Prado: gossypetin is the best substrate, followed by quercetin, kaempferol and myricetin, cv. Stern des Orients: 3times lower specific activity than with gossypetin as substrate | Glebionis segetum | UDP + quercetin 7-O-beta-D-glucoside | - |
? | |
| UDP-glucose + a flavonol | - |
Glebionis segetum | UDP + a flavonol 7-O-beta-D-glucoside | - |
? | |
| UDP-glucose + apigenin | cv. Prado | Glebionis segetum | UDP + apigenin 7-O-beta-D-glucoside | - |
? | |
| UDP-glucose + eriodictyol | cv. Prado | Glebionis segetum | UDP + eriodictyol 7-O-beta-D-glucoside | - |
? | |
| UDP-glucose + gossypetin | gossypetin and quercetin are presumably the natural substrates | Glebionis segetum | UDP + gossypetin 7-O-beta-D-glucoside | gossypetin 7-O-glucoside is particularly responsible for the yellow coloration of the petals | ? | |
| UDP-glucose + gossypetin | cv. Prado: best substrate, followed by quercetin, kaempferol and myricetin, cv. Stern des Orients: 3times higher specific activity than with quercetin as substrate | Glebionis segetum | UDP + gossypetin 7-O-beta-D-glucoside | - |
? | |
| UDP-glucose + luteolin | cv. Prado | Glebionis segetum | UDP + luteolin 7-O-beta-D-glucoside | - |
? | |
| UDP-glucose + myricetin | cv. Prado, gossypetin is the best substrate, followed by quercetin, kaempferol and myricetin | Glebionis segetum | UDP + myricetin 7-O-beta-D-glucoside | - |
? | |
| UDP-glucose + naringenin | cv. Prado | Glebionis segetum | UDP + naringenin 7-O-beta-D-glucoside | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| UDP-glucose:flavonol 7-O-glucosyltransferase | - |
Glebionis segetum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Glebionis segetum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
- |
7-O-glucosylation also proceeds at low temperatures, e.g. 0°C | Glebionis segetum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
10 min, no influence on the reaction up to 40°C, higher temperatures lead to loss of activity | Glebionis segetum |
| 60 | - |
10 min, incubation without UDP-glucose, complete loss of activity | Glebionis segetum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
the pH-optimum is highly dependent on the substrate used | Glebionis segetum |
| 6.9 | - |
gossypetin or naringenin as substrate | Glebionis segetum |
| 7.5 | - |
eriodictyol as substrate | Glebionis segetum |
| 7.7 | - |
apigenin as substrate | Glebionis segetum |
| 7.8 | - |
kaempferol as substrate | Glebionis segetum |
| 8.1 | - |
myricetin or luteolin as substrate | Glebionis segetum |
| 8.3 | - |
quercetin as substrate | Glebionis segetum |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
