Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme expression is inducible by plant hormones, such as salicylic acid, auxin, 2,4-dichorophenoxyacetic acid, and jasmonate | Nicotiana tabacum |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Nicotiana tabacum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0065 | - |
kaempferol | pH 8.0, 30°C, recombinant enzyme | Nicotiana tabacum | |
0.0236 | - |
3-hydroxycoumarin | pH 8.0, 30°C, recombinant enzyme | Nicotiana tabacum | |
0.0432 | - |
UDP-glucose | pH 8.0, 30°C, recombinant enzyme | Nicotiana tabacum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Nicotiana tabacum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Nicotiana tabacum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | broad substrate specificity, no activity with salicylic acid, cyanidin, and cyanidin 3-O-glucoside, poor activity with 4-hydroxycoumarin and 5-hydroxyflavone | Nicotiana tabacum | ? | - |
? | |
UDP-alpha-D-glucose + kaempferol | preferred substrate | Nicotiana tabacum | UDP + kaempferol 7-O-beta-D-glucoside | - |
? | |
UDP-alpha-D-glucose + quercetin | 19% of the activity with kaempferol | Nicotiana tabacum | UDP + quercetin 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + 1-naphthol | 44% of the activity with kaempferol | Nicotiana tabacum | UDP + naphthol 1-O-beta-D-glucoside | - |
? | |
UDP-glucose + 2-coumaric acid | 41% of the activity with kaempferol | Nicotiana tabacum | UDP + ? | - |
? | |
UDP-glucose + 2-naphthol | 14% of the activity with kaempferol | Nicotiana tabacum | UDP + naphthol 2-O-beta-D-glucoside | - |
? | |
UDP-glucose + 3-hydroxycoumarin | 310% of the activity with kaempferol | Nicotiana tabacum | UDP + coumarin 3-O-beta-D-glucoside | - |
? | |
UDP-glucose + 3-hydroxyflavone | 12% of the activity with kaempferol | Nicotiana tabacum | UDP + 3-hydroxyflavone 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + 4-coumaric acid | 43% of the activity with kaempferol | Nicotiana tabacum | UDP + ? | - |
? | |
UDP-glucose + 6-hydroxyflavone | 24% of the activity with kaempferol | Nicotiana tabacum | UDP + 6-hydroxyflavone 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + 7-hydroxyflavone | 53% of the activity with kaempferol | Nicotiana tabacum | UDP + 7-hydroxyflavone 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + esculetin | 24% of the activity with kaempferol | Nicotiana tabacum | UDP + esculetin 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + kaempferol 3-O-glucoside | 45% of the activity with kaempferol | Nicotiana tabacum | UDP + kaempferol 3,7beta-O-diglucoside | - |
? | |
UDP-glucose + scopoletin | 11% of the activity with kaempferol | Nicotiana tabacum | UDP + scopoletin 7-O-beta-D-glucoside | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GT2 | - |
Nicotiana tabacum |
NTGT2 | - |
Nicotiana tabacum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Nicotiana tabacum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
with kaempferol as substrate | Nicotiana tabacum |