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Literature summary for 2.4.1.242 extracted from
- Granule-bound starch synthase I. A major enzyme involved in the biogenesis of B-crystallites in starch granules (2002), Eur. J. Biochem., 269, 3810-3820.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| GBSSI, DNA sequence determination and analysis, expression in Escherichia coli, complementation of the sta2-1 mutation, phylogenetic tree | Chlamydomonas reinhardtii |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | the sta2-29::ARG7 mutation causes loss of GBSSI activity, the Sta2-1 mutation results in production of a truncated 58 kDa GBSSI isozyme | Chlamydomonas reinhardtii |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
x * 76000, immature wild-type GBSSI, SDS-PAGE, x * 69000, mature wild-type GBSSI, SDS-PAGE, x * 58000, truncated mutant sta2-1, SDS-PAGE | Chlamydomonas reinhardtii |
| 69000 | - |
x * 76000, immature wild-type GBSSI, SDS-PAGE, x * 69000, mature wild-type GBSSI, SDS-PAGE, x * 58000, truncated mutant sta2-1, SDS-PAGE | Chlamydomonas reinhardtii |
| 76000 | - |
x * 76000, immature wild-type GBSSI, SDS-PAGE, x * 69000, mature wild-type GBSSI, SDS-PAGE, x * 58000, truncated mutant sta2-1, SDS-PAGE | Chlamydomonas reinhardtii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP-glucose + (1,4-alpha-glucosyl)n | Chlamydomonas reinhardtii | i.e. starch, composition of starch granule, including amylopectin, amylose, A-type and B-type crystals | ADP + (1,4-alpha-glucosyl)n+1 | - |
? |  |
| additional information | Chlamydomonas reinhardtii | decreased amylose synthesis activity and altered starch granule morphology during nitrogen starvation, overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chlamydomonas reinhardtii | O64925 | complete cDNA | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | the transit peptide of the immature isozyme GBSSI is cleaved off resulting in the mature enzyme | Chlamydomonas reinhardtii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP-glucose + (1,4-alpha-glucosyl)n | i.e. starch, composition of starch granule, including amylopectin, amylose, A-type and B-type crystals | Chlamydomonas reinhardtii | ADP + (1,4-alpha-glucosyl)n+1 | - |
? | |
| ADP-glucose + (1,4-alpha-glucosyl)n | i.e. starch | Chlamydomonas reinhardtii | ADP + (1,4-alpha-glucosyl)n+1 | - |
? | |
| additional information | decreased amylose synthesis activity and altered starch granule morphology during nitrogen starvation, overview | Chlamydomonas reinhardtii | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 76000, immature wild-type GBSSI, SDS-PAGE, x * 69000, mature wild-type GBSSI, SDS-PAGE, x * 58000, truncated mutant sta2-1, SDS-PAGE | Chlamydomonas reinhardtii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GBSSI | - |
Chlamydomonas reinhardtii |
| granule-bound starch synthase I | - |
Chlamydomonas reinhardtii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Chlamydomonas reinhardtii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
assay at | Chlamydomonas reinhardtii |
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Release 2023.1 (January 2023)
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