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Literature summary for 2.4.1.242 extracted from
- Biochemical evidence for the role of the waxy protein from pea (Pisum sativum L.) as a granule-bound starch synthase (1993), Plant Physiol., 103, 1355-1359.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | 60000 | sucrose density gradient centrifugation | Pisum sativum |
| 60000 | - |
1 * 60000, SDS-PAGE | Pisum sativum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP-glucose + (1,4-alpha-glucosyl)n | Pisum sativum | the Waxy protein and the granule-bound starch synthase are identical | ADP + (1,4-alpha-glucosyl)n+1 | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pisum sativum | - |
round-seeded var. Alaska, 2 isozymes | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partially from lyophilized starch extracted from developing embryos, by anion exchange chromatography | Pisum sativum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| embryo | developing | Pisum sativum | - |
| seed | starch granule | Pisum sativum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10 | - |
partially purified isozyme I | Pisum sativum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP-glucose + (1,4-alpha-glucosyl)n | - |
Pisum sativum | ADP + (1,4-alpha-glucosyl)n+1 | - |
? | |
| ADP-glucose + (1,4-alpha-glucosyl)n | the Waxy protein and the granule-bound starch synthase are identical | Pisum sativum | ADP + (1,4-alpha-glucosyl)n+1 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 60000, SDS-PAGE | Pisum sativum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GBSSI | - |
Pisum sativum |
| granule-bound starch synthase | - |
Pisum sativum |
| Waxy protein | - |
Pisum sativum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Pisum sativum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.4 | - |
assay at | Pisum sativum |
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Release 2023.1 (January 2023)
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