Activating Compound | Comment | Organism | Structure |
---|---|---|---|
citrate | stimulates the UDP-glucose utilizing enzyme | Agarophyton tenuistipitatum | |
UDP | product inhibition of the UDP-glucose utilizing enzyme | Agarophyton tenuistipitatum |
General Stability | Organism |
---|---|
rapid loss of activity of purified UDP-glucose utilizing enzyme within hours | Agarophyton tenuistipitatum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | UDP-glucose utilizing activity is sensitive to proteolytic inhibition during extraction, while the ADP-glucose utilizing activity is unaffected | Agarophyton tenuistipitatum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Agarophyton tenuistipitatum | |
0.17 | - |
glycogen | UDP-glucose utilizing enzyme in presence of 0.5 M citrate | Agarophyton tenuistipitatum | |
0.52 | - |
amylopectin | UDP-glucose utilizing enzyme in presence of 0.5 M citrate | Agarophyton tenuistipitatum | |
0.69 | - |
glycogen | UDP-glucose utilizing enzyme in absence of citrate | Agarophyton tenuistipitatum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | starch granule-bound | Agarophyton tenuistipitatum | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
145000 | - |
4 * 145000, UDP-glucose utilizing enzyme, SDS-PAGE | Agarophyton tenuistipitatum |
580000 | - |
UDP-glucose utilizing enzyme, gel filtration | Agarophyton tenuistipitatum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-glucose + (1,4-alpha-glucosyl)n | Agarophyton tenuistipitatum | ADP-glucose is almost 9fold less effective than UDP-glucose, utilized by the ADP-glucose utilizing enzyme | ADP + (1,4-alpha-glucosyl)n+1 | - |
? | |
UDP-glucose + (1,4-alpha-glucosyl)n | Agarophyton tenuistipitatum | UDP-glucose is the preferred glucosyl donor, utilized by the UDP-glucose utilizing enzyme | UDP + (1,4-alpha-glucosyl)n+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agarophyton tenuistipitatum | - |
red alga | - |
Oxidation Stability | Organism |
---|---|
UDP-glucose utilizing activity is sensitive to oxidative inhibition during extraction, while the ADP-glucose utilizing activity is unaffected | Agarophyton tenuistipitatum |
Purification (Comment) | Organism |
---|---|
ADP-glucose utilizing activity partially, UDP-glucose utilizing activity 368.7fold to homogeneity | Agarophyton tenuistipitatum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
UDP-glucose utilizing and ADP-glucose utilizing activities in different cell fractions and with different primer substrate, overview | Agarophyton tenuistipitatum |
0.229 | - |
purified UDP-glucose utilizing enzyme | Agarophyton tenuistipitatum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-glucose + (1,4-alpha-glucosyl)n | ADP-glucose is almost 9fold less effective than UDP-glucose, utilized by the ADP-glucose utilizing enzyme | Agarophyton tenuistipitatum | ADP + (1,4-alpha-glucosyl)n+1 | - |
? | |
ADP-glucose + (1,4-alpha-glucosyl)n | ADP-glucose is almost 9fold less effective than UDP-glucose, utilized by the ADP-glucose utilizing enzyme and UDP-glucose utilizing enzyme, the latter shows 3.8% activity compared to UDP-glucose | Agarophyton tenuistipitatum | ADP + (1,4-alpha-glucosyl)n+1 | - |
? | |
ADP-glucose + amylopectin | - |
Agarophyton tenuistipitatum | ADP + ? | - |
? | |
CDP-glucose + (1,4-alpha-glucosyl)n | UDP-glucose utilizing enzyme, 2.5% of the activity with UDP-glucose | Agarophyton tenuistipitatum | CDP + ? | - |
? | |
GDP-glucose + (1,4-alpha-glucosyl)n | UDP-glucose utilizing enzyme, 28.7% of the activity with UDP-glucose | Agarophyton tenuistipitatum | GDP + ? | - |
? | |
additional information | the UDP-glucose utilizing enzyme shows broad primer specificity | Agarophyton tenuistipitatum | ? | - |
? | |
NDP-glucose + glycogen | - |
Agarophyton tenuistipitatum | ? | - |
? | |
UDP-galactose + (1,4-alpha-glucosyl)n | UDP-glucose utilizing enzyme, 10.1% of the activity with UDP-glucose | Agarophyton tenuistipitatum | UDP + ? | - |
? | |
UDP-glucose + (1,4-alpha-glucosyl)n | UDP-glucose is the preferred glucosyl donor, utilized by the UDP-glucose utilizing enzyme | Agarophyton tenuistipitatum | UDP + (1,4-alpha-glucosyl)n+1 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 145000, UDP-glucose utilizing enzyme, SDS-PAGE | Agarophyton tenuistipitatum |
Synonyms | Comment | Organism |
---|---|---|
UDPGlc: starch synthase | - |
Agarophyton tenuistipitatum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.7 | 8.1 | with substrate glycogen | Agarophyton tenuistipitatum |
8.1 | - |
with substrate amylopectin | Agarophyton tenuistipitatum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | with substrate amylopectin, sharp drop of activity below pH 6.5 and more gradually above pH 8.0 | Agarophyton tenuistipitatum |
7 | 9 | with substrate amylopectin, sharp drop of activity below pH 7.0 and above pH 9.0 | Agarophyton tenuistipitatum |