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Literature summary for 2.4.1.248 extracted from

  • Watanabe, H.; Nishimoto, T.; Chaen, H.; Fukuda, S.
    A novel glucanotransferase that produces a cyclomaltopentaose cyclized by an alpha-1,6-linkage (2007), J. Appl. Glycosci., 54, 109-118.
No PubMed abstract available
Search for more literature for 2.4.1.248

Activating Compound

Activating Compound Comment Organism Structure
Ca2+ enhances thermal stability Niallia circulans

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM, 80% residual acitvity Niallia circulans
EDTA 1 mM, 86% residual acitvity Niallia circulans
Fe2+ 1 mM, 94% residual acitvity Niallia circulans
Fe3+ 1 mM, 82% residual acitvity Niallia circulans
Hg2+ 1 mM, 55% residual acitvity Niallia circulans

Metals/Ions

Metals/Ions Comment Organism Structure
Pb2+ 1 mM, 130% of initial activity Niallia circulans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
106000
-
 2 * 106000, SDS-PAGE Niallia circulans
184000
-
 gel filtration Niallia circulans

Organism

Organism UniProt Comment Textmining
Niallia circulans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Niallia circulans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1
-
 pH 6.0, 45 Niallia circulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylose
-
 Niallia circulans cyclo-(-6)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-) + ? yield 27.2% ?
glycogen
-
 Niallia circulans cyclo-(-6)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-) + ? yield 14.6% ?
maltoheptaose
-
 Niallia circulans cyclo-(-6)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-) + ? yield 15.8% ?
maltohexaose
-
 Niallia circulans cyclo-(-6)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-) + ? yield 12.7% ?
maltopentaose
-
 Niallia circulans cyclo-(-6)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-) + ? yield 8.9% ?
maltotetraose
-
 Niallia circulans cyclo-(-6)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-) + ? yield 4.3% ?
maltotriose poor substrate Niallia circulans cyclo-(-6)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-) + ? yield 0.9% ?
additional information enzyme acts on maltooligosaccharides that have degrees of polymerization of 3 or more, amylose, and soluble starch, to produce cyclo-(-6)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-) by an intramolecular alpha-1,6-glycosyl transfer reaction. Enzyme additionally catalyzes the transfer of part of a linear oligosaccharide to another oligosaccharide by an intermolecular alpha-1,4-glycosyl transfer reaction Niallia circulans ?
-
 ?
soluble starch
-
 Niallia circulans cyclo-(-6)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-) + ? yield 26.5% ?

Subunits

Subunits Comment Organism
dimer 2 * 106000, SDS-PAGE Niallia circulans

Synonyms

Synonyms Comment Organism
isocyclomaltooligosaccharide glucanotransferase
-
 Niallia circulans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Niallia circulans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 stable up to Niallia circulans
40
-
 in presence of 1 mM Ca2+, stable up to Niallia circulans

pH Range

pH Minimum pH Maximum Comment Organism
4.5 8
-
 Niallia circulans

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 9
-
 Niallia circulans

pI Value

Organism Comment pI Value Maximum pI Value
Niallia circulans isoelectric focusing
-
 7.5