Any feedback?
Literature summary for 2.4.1.25 extracted from
- Thermus aquaticus ATCC 33923 amylomaltase gene cloning and expression and enzyme characterization: production of cycloamylose (1999), Appl. Environ. Microbiol., 65, 910-915.
Application
| Application | Comment | Organism |
|---|---|---|
| nutrition | cycloamylose will be used in the food, pharmaceutical and chemical industries | Thermus aquaticus |
| synthesis | cycloamylose will be used in the food, pharmaceutical and chemical industries | Thermus aquaticus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Escherichia coli |
- |
Solanum tuberosum |
- |
Streptococcus pneumoniae |
- |
Clostridium butyricum |
- |
Chlamydia psittaci |
- |
Thermus aquaticus |
| cloned and sequenced and overexpressed in Escherichia coli | Thermus aquaticus |
| putative gene identified in the genome | Mycobacterium tuberculosis |
| putative gene identified in the genome | Borreliella burgdorferi |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57220 | - |
amino acid sequence | Thermus aquaticus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aquifex aeolicus | - |
- |
- |
| Borreliella burgdorferi | - |
- |
- |
| Chlamydia psittaci | - |
- |
- |
| Clostridium butyricum | - |
NCIMB 7423 | - |
| Clostridium butyricum NCIMB 7423 | - |
NCIMB 7423 | - |
| Escherichia coli | - |
- |
- |
| Haemophilus influenzae | - |
- |
- |
| Hordeum vulgare | - |
barley | - |
| Mycobacterium tuberculosis | - |
- |
- |
| Solanum tuberosum | - |
potato | - |
| Streptococcus pneumoniae | - |
- |
- |
| Synechocystis sp. | - |
- |
- |
| Thermococcus litoralis | - |
- |
- |
| Thermus aquaticus | - |
ATCC 33923 | - |
| Thermus aquaticus | B7A9X4 | ATCC 33923, nucleotide sequence accession number, deposited in DDBJ/EMBL/GenBank | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Hordeum vulgare |
- |
Thermococcus litoralis |
| native and recombinant enzyme | Thermus aquaticus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| tuber | - |
Solanum tuberosum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.9 | - |
- |
Thermus aquaticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,4-alpha-D-glucan + 1,4-alpha-D-glucan | - |
Thermus aquaticus | maltooligosaccharides | - |
? |  |
| 1,4-alpha-D-glucan + 1,4-alpha-D-glucan | - |
Escherichia coli | maltooligosaccharides | - |
? | |
| 1,4-alpha-D-glucan + 1,4-alpha-D-glucan | - |
Solanum tuberosum | maltooligosaccharides | - |
? | |
| 1,4-alpha-D-glucan + glucose | - |
Thermus aquaticus | maltooligosaccharides | - |
? | |
| 1,4-alpha-D-glucan + glucose | - |
Escherichia coli | maltooligosaccharides | - |
? | |
| 1,4-alpha-D-glucan + glucose | - |
Solanum tuberosum | maltooligosaccharides | - |
? | |
| amylose + maltopentaose | - |
Thermus aquaticus | cyclic alpha-1,4-glucan | cycloamylose | ? | |
| amylose + maltopentaose | - |
Solanum tuberosum | cyclic alpha-1,4-glucan | cycloamylose | ? | |
| amylose + maltopentaose | - |
Thermococcus litoralis | cyclic alpha-1,4-glucan | cycloamylose | ? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
- |
Thermus aquaticus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
stable up to, retains 50% activity after incubation at 80°C for 24 h, loses all activity after incubation at 100°C for 10 min | Thermus aquaticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 6 | - |
Thermus aquaticus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | - |
Thermus aquaticus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
