Cloned (Comment) | Organism |
---|---|
genes of wild-type and mutant enzymes are coexpressed in Escherichia coli cells with GroELS, tRNA(AGA), and tRNA(AGG) | Thermococcus litoralis |
Protein Variants | Comment | Organism |
---|---|---|
E123Q | the specific activity of the mutant enzyme toward maltotriose is about 15000fold lower than the specific activity of the wild-type enzyme | Thermococcus litoralis |
E129Q | the specific activity of the mutant enzyme is almost the same as that of the wild-type enzyme | Thermococcus litoralis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus litoralis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermococcus litoralis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-chloro-4-nitrophenyl 4'''',6''''-O-(3-oxobutylidene)maltopentaoside + D-glucose | the disproportionation reaction of the enzyme involves a ping-pong bi-bi mechanism. On the basis of this reaction mechanism, the glycosyl-enzyme intermediate, in which a donor substrate is covalently bound to the catalytic nucleophile, is trapped by treating the enzyme with 3-ketobutylidene-beta-2-chloro-4-nitrophenyl maltopentaoside in the absence of an acceptor and is detected by matrix-assisted laser desorption ionization time-of-flight mass spectrometry after peptic digestion | Thermococcus litoralis | ? | - |
? | |
maltotriose + maltotriose | - |
Thermococcus litoralis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TLGT | - |
Thermococcus litoralis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
assay at | Thermococcus litoralis |