Cloned (Comment) | Organism |
---|---|
expressed in fusion with maltose-binding protein in Escherichia coli BL21(DE3) cells and as His-tagged protein in Rosetta(DE3)pLysS cells | Xylella fastidiosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
1 * 45000, dynamic light scattering | Xylella fastidiosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xylella fastidiosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
maltose-binding-protein-fused enzyme is partially purified by amylose column chromatography, His-tagged enzyme is purified by Ni-NTA column chromatography | Xylella fastidiosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-mannose + cellobiose-diphosphate-polyprenol carrier lipid | GumH enzyme is an alpha-1,3-mannosyltransferase responsible for the transfer of mannose from GDP-mannose to the cellobiose-diphosphate-polyprenol carrier lipid during the assembly of the tetrasaccharide repeat unit of the exopolysaccharide fastidian gum | Xylella fastidiosa | GDP + D-mannose-alpha-(1-3)-cellobiose-diphosphate-polyprenol carrier lipid | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 45000, dynamic light scattering | Xylella fastidiosa |
Synonyms | Comment | Organism |
---|---|---|
GDP-mannosyltransferase | - |
Xylella fastidiosa |
gumH | - |
Xylella fastidiosa |
General Information | Comment | Organism |
---|---|---|
physiological function | GumH enzyme is an alpha-1,3-mannosyltransferase responsible for the transfer of mannose from GDP-mannose to the cellobiose-diphosphate-polyprenol carrier lipid during the assembly of the tetrasaccharide repeat unit of the exopolysaccharide fastidian gum | Xylella fastidiosa |