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Literature summary for 2.4.1.255 extracted from
- A modified coupled enzyme method for O-linked GlcNAc transferase activity assay (2009), Biol. Proced. Online, 11, 170-183.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| full length and functional human mOGT is difficult to be expressed in Escherichia coli, truncated mOGT recombinant (C-654) is constructed and expressed | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.16 | - |
YSDSPSTST | - |
Homo sapiens |  |
| 0.17 | - |
UDP-GlcNAc | - |
Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 73000 | - |
theoretical | Homo sapiens |
| 75000 | - |
highly purified C-654, truncated mOGT recombinant | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Homo sapiens | transfer of O-linked GlcNAc to serine/threonine residues of a variety of substrate proteins, including nuclear pore proteins, transcription factors, and proteins implicated in diabetes and neurodegenerative disorders | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| additional information | enzyme for protein post-translational modifications | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni2+-affinity chromatography | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | transfer of O-linked GlcNAc to serine/threonine residues of a variety of substrate proteins, including nuclear pore proteins, transcription factors, and proteins implicated in diabetes and neurodegenerative disorders | Homo sapiens | ? | - |
? | |
| UDP-GlcNAc + YSDSPSTST | coupled enzyme assay of C-654 | Homo sapiens | YSDSP-(GlcNAc)STST + UDP | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| mOGT | - |
Homo sapiens |
| O-linked GlcNAc transferase | - |
Homo sapiens |
| OGT | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.76 | - |
UDP-GlcNAc | - |
Homo sapiens | |
| 2.48 | - |
YSDSPSTST | - |
Homo sapiens | |
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Release 2023.1 (January 2023)
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