Cloned (Comment) | Organism |
---|---|
ncOGT, mOGT, and sOGT constructs are cloned into a modified pET-24b vector for expression as C-terminal His8 fusions. Expression in Escherichia coli | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(2Z)-2-[(4-chlorophenyl)imino]-4-oxo-3-(2-tricyclo[3.3.1.1(3,7)]dec-1-ylethyl)-1,3-thiazinane-6-carboxylic acid | donor analogue displacement probes | Homo sapiens | |
3-(4-cyanobenzylthio)-1-(thiophen-2-yl)-5,6,7,8-tetrahydroisoquinoline-4-carboxylic acid | donor analogue displacement probes | Homo sapiens | |
phenyl 6-chloro-2-oxobenzo[d]oxazole-3(2H)-carboxylate | donor analogue displacement probes | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0067 | - |
UDP-GlcNAc | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | enzyme transfers N-acetylglucosamine from UDP-GlcNAc to selected serine and threonine residues | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.161 | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme transfers N-acetylglucosamine from UDP-GlcNAc to selected serine and threonine residues | Homo sapiens | ? | - |
? | |
UDP-GlcNAc + Nup62 protein | - |
Homo sapiens | UDP + N-acetyl-D-glucosmainyl-[Nup62 protein] | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | OGT is a bipartite protein consisting of a C-terminal glycosyltransferase (Gtf) domain and an N-terminal protein-protein interaction domain comprised of 12 tetratricopeptide (TPR) repeats | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
mOGT | splice variant | Homo sapiens |
ncOGT | splice variant | Homo sapiens |
O-GlcNAc transferase | - |
Homo sapiens |
OGT | - |
Homo sapiens |
sOGT | splice variant | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
additional information | - |
for ncOGT IC50 value is about 100-150 microM | Homo sapiens | 3-(4-cyanobenzylthio)-1-(thiophen-2-yl)-5,6,7,8-tetrahydroisoquinoline-4-carboxylic acid | |
0.01 | - |
ncOGT | Homo sapiens | phenyl 6-chloro-2-oxobenzo[d]oxazole-3(2H)-carboxylate | |
0.027 | - |
sOGT | Homo sapiens | phenyl 6-chloro-2-oxobenzo[d]oxazole-3(2H)-carboxylate | |
0.03 | - |
sOGT | Homo sapiens | (2Z)-2-[(4-chlorophenyl)imino]-4-oxo-3-(2-tricyclo[3.3.1.1(3,7)]dec-1-ylethyl)-1,3-thiazinane-6-carboxylic acid | |
0.053 | - |
ncOGT | Homo sapiens | (2Z)-2-[(4-chlorophenyl)imino]-4-oxo-3-(2-tricyclo[3.3.1.1(3,7)]dec-1-ylethyl)-1,3-thiazinane-6-carboxylic acid | |
0.06 | - |
sOGT | Homo sapiens | 3-(4-cyanobenzylthio)-1-(thiophen-2-yl)-5,6,7,8-tetrahydroisoquinoline-4-carboxylic acid |
General Information | Comment | Organism |
---|---|---|
metabolism | O-GlcN acylation affects such diverse cellular processes as transcription, translation, organelle targeting, and protein-protein interactions,1 and is believed to play a role in a variety of signaling cascades that mediate glucose homeostasis and stress responses | Homo sapiens |