Crystallization (Comment) | Organism |
---|---|
in complex with UDP and GlcNAc. GtfA reveals a beta-meander add-on domain beyond the catalytic domain, which is distinct from the all-alpha-tetratricopeptide repeats in the two structure-known OGTs. This add-on domain contributes to forming an active GtfA-GtfB complex and recognizing the acceptor protein | Streptococcus pneumoniae TIGR4 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
UDP-GlcNAc | GtfA-GtfB complex, pH 7.5, 37°C | Streptococcus pneumoniae TIGR4 | |
11.8 | - |
UDP-GlcNAc | isolated core enzyme GtfA, pH 7.5, 37°C | Streptococcus pneumoniae TIGR4 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pneumoniae TIGR4 | A0A0H2URG7 | - |
- |
Streptococcus pneumoniae TIGR4 ATCC BAA-334 | A0A0H2URG7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adhesin PsrP + UDP-GlcNAc | - |
Streptococcus pneumoniae TIGR4 | ? + UDP | the core enzyme GtfA and co-activator GtfB form an OGT complex to glycosylate the first serine-rich repeat of adhesin PsrP (pneumococcal serine-rich repeat protein), which is involved in the infection and pathogenesis. Formation of the GtfA-GtfB complex is crucial for the O-GlcNAcylation activity | ? | |
adhesin PsrP + UDP-GlcNAc | - |
Streptococcus pneumoniae TIGR4 ATCC BAA-334 | ? + UDP | the core enzyme GtfA and co-activator GtfB form an OGT complex to glycosylate the first serine-rich repeat of adhesin PsrP (pneumococcal serine-rich repeat protein), which is involved in the infection and pathogenesis. Formation of the GtfA-GtfB complex is crucial for the O-GlcNAcylation activity | ? | |
UDP-GlcNAc + H2O | - |
Streptococcus pneumoniae TIGR4 | UDP + GlcNAc | - |
? | |
UDP-GlcNAc + H2O | - |
Streptococcus pneumoniae TIGR4 ATCC BAA-334 | UDP + GlcNAc | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Gtf1 | - |
Streptococcus pneumoniae TIGR4 |
GtfA | - |
Streptococcus pneumoniae TIGR4 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.122 | - |
UDP-GlcNAc | isolated core enzyme GtfA, pH 7.5, 37°C | Streptococcus pneumoniae TIGR4 | |
1.25 | - |
UDP-GlcNAc | GtfA-GtfB complex, pH 7.5, 37°C | Streptococcus pneumoniae TIGR4 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
UDP-GlcNAc | isolated core enzyme GtfA, pH 7.5, 37°C | Streptococcus pneumoniae TIGR4 | |
1.15 | - |
UDP-GlcNAc | GtfA-GtfB complex, pH 7.5, 37°C | Streptococcus pneumoniae TIGR4 |