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Literature summary for 2.4.1.255 extracted from

  • Shi, W.W.; Jiang, Y.L.; Zhu, F.; Yang, Y.H.; Shao, Q.Y.; Yang, H.B.; Ren, Y.M.; Wu, H.; Chen, Y.; Zhou, C.Z.
    Structure of a novel O-linked N-acetyl-D-glucosamine (O-GlcNAc) transferase, GtfA, reveals insights into the glycosylation of pneumococcal serine-rich repeat adhesins (2014), J. Biol. Chem., 289, 20898-20907.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with UDP and GlcNAc. GtfA reveals a beta-meander add-on domain beyond the catalytic domain, which is distinct from the all-alpha-tetratricopeptide repeats in the two structure-known OGTs. This add-on domain contributes to forming an active GtfA-GtfB complex and recognizing the acceptor protein Streptococcus pneumoniae TIGR4

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1
-
UDP-GlcNAc GtfA-GtfB complex, pH 7.5, 37°C Streptococcus pneumoniae TIGR4
11.8
-
UDP-GlcNAc isolated core enzyme GtfA, pH 7.5, 37°C Streptococcus pneumoniae TIGR4

Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae TIGR4 A0A0H2URG7
-
-
Streptococcus pneumoniae TIGR4 ATCC BAA-334 A0A0H2URG7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adhesin PsrP + UDP-GlcNAc
-
Streptococcus pneumoniae TIGR4 ? + UDP the core enzyme GtfA and co-activator GtfB form an OGT complex to glycosylate the first serine-rich repeat of adhesin PsrP (pneumococcal serine-rich repeat protein), which is involved in the infection and pathogenesis. Formation of the GtfA-GtfB complex is crucial for the O-GlcNAcylation activity ?
adhesin PsrP + UDP-GlcNAc
-
Streptococcus pneumoniae TIGR4 ATCC BAA-334 ? + UDP the core enzyme GtfA and co-activator GtfB form an OGT complex to glycosylate the first serine-rich repeat of adhesin PsrP (pneumococcal serine-rich repeat protein), which is involved in the infection and pathogenesis. Formation of the GtfA-GtfB complex is crucial for the O-GlcNAcylation activity ?
UDP-GlcNAc + H2O
-
Streptococcus pneumoniae TIGR4 UDP + GlcNAc
-
?
UDP-GlcNAc + H2O
-
Streptococcus pneumoniae TIGR4 ATCC BAA-334 UDP + GlcNAc
-
?

Synonyms

Synonyms Comment Organism
Gtf1
-
Streptococcus pneumoniae TIGR4
GtfA
-
Streptococcus pneumoniae TIGR4

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.122
-
UDP-GlcNAc isolated core enzyme GtfA, pH 7.5, 37°C Streptococcus pneumoniae TIGR4
1.25
-
UDP-GlcNAc GtfA-GtfB complex, pH 7.5, 37°C Streptococcus pneumoniae TIGR4

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.011
-
UDP-GlcNAc isolated core enzyme GtfA, pH 7.5, 37°C Streptococcus pneumoniae TIGR4
1.15
-
UDP-GlcNAc GtfA-GtfB complex, pH 7.5, 37°C Streptococcus pneumoniae TIGR4