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Literature summary for 2.4.1.26 extracted from

  • Kornberg, S.R.; Zimmerman, S.B.; Kornberg, A.
    Glucosylation of deoxyribonucleic acid by enzymes from bacteriophage-infected Escherichia coli (1961), J. Biol. Chem., 236, 1487-1493.
    View publication on PubMed
Search for more literature for 2.4.1.26

Activating Compound

Activating Compound Comment Organism Structure
phosphate buffer stimulates Tequatrovirus T4
sulfhydryl reagents required Enterobacteria phage T6

Metals/Ions

Metals/Ions Comment Organism Structure
MgCl2
-
 Enterobacteria phage T6
MgCl2 stimulates Tequatrovirus T4

Organism

Organism UniProt Comment Textmining
Enterobacteria phage T6
-
 Escherichia coli infected with
-
Escherichia phage T2
-
 Escherichia coli infected with
-
Tequatrovirus T4
-
 Escherichia coli infected with
-

Purification (Commentary)

Purification (Comment) Organism
partial, E. coli infected with Tequatrovirus T4
partial, E. coli infected with Escherichia phage T2
partial, E. coli infected with Enterobacteria phage T6

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Tequatrovirus T4
additional information
-
-
 Escherichia phage T2
additional information
-
-
 Enterobacteria phage T6

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDPglucose + 5-hydroxymethylcytosine containing DNA
-
 Escherichia phage T2 UDP + alpha-glucosyl-5-hydroxymethylcytosine containing DNA
-
 ?
UDPglucose + 5-hydroxymethylcytosine containing DNA extent of glucosylation varies between 50% and 80% of the amount of hydroxymethylcytosine residues calculated to be present, T4 DNA with no unsubstituted hydroxymethylcytosine groups does not serve as acceptor, T2 DNA: no activity with enzyme from T2-infected cells, small activity with enzyme from T4- and T6-infected cells Tequatrovirus T4 UDP + alpha-glucosyl-5-hydroxymethylcytosine containing DNA
-
 ?
UDPglucose + 5-hydroxymethylcytosine containing DNA extent of glucosylation varies between 50% and 80% of the amount of hydroxymethylcytosine residues calculated to be present, T4 DNA with no unsubstituted hydroxymethylcytosine groups does not serve as acceptor, T2 DNA: no activity with enzyme from T2-infected cells, small activity with enzyme from T4- and T6-infected cells Enterobacteria phage T6 UDP + alpha-glucosyl-5-hydroxymethylcytosine containing DNA
-
 ?